华北农学报2017，Vol.32Issue(4)：67-72,6.DOI:10.7668/hbnxb.2017.04.011
高温条件下CO2对黄瓜SOD、POD和CAT活性及其基因表达的影响
Effect of CO2 on Cucumber Leaf SOD,POD and CAT Activity and Gene Expression under High Temperature
摘要
Abstract
In order to explore the effect of CO2 on SOD, POD and CAT activity and their gene expressions under high temperature, the grafting cucumber in greenhouse was used as material, trying to study the changes of cucumber leaf SOD, POD and CAT activity and gene expression under high temperature after treating with CO2.The results showed SOD,POD and CAT activity in cucumber leaf were increased after treating with CO2 under high temperature.Comparing with room temperature treatment,the max difference of cucumber SOD,POD and CAT activity were at 42,28 and 21 days after treating with CO2 and high temperature,and increased by 19.1%,50.4% and 45.0% respectively.Using Real-time PCR analysis cucumber SOD,POD and CAT gene expression were all increased after treating with CO2 under high temperature.The max difference of cucumber SOD,POD and CAT gene expression level were at 28,28 and 21 days after treating with CO2 and high temperature,and with 60.7%,70.3% and 44.9% higher than room temperature treatment respectively.In conclusion,after treating with CO2,the cucumber SOD,POD and CAT activity and their gene expression level were both increased under high temperature.关键词
黄瓜/高温/CO2/抗氧化酶/基因表达Key words
Cucumber/High temperature/CO2/Antioxidase/Gene expression分类
农业科技引用本文复制引用
张之为,李晓静,白金瑞,陈帅,范梦轩..高温条件下CO2对黄瓜SOD、POD和CAT活性及其基因表达的影响[J].华北农学报,2017,32(4):67-72,6.基金项目
内蒙古教育厅项目(NJZY14083) （NJZY14083）
内蒙古自然科学基金项目(2015BS0313) （2015BS0313）
