华北农学报2017，Vol.32Issue(4)：78-84,7.DOI:10.7668/hbnxb.2017.04.013
中国水仙凝集素基因在大花烟草中的定量表达及抗蚜性分析
Analysis of qPCR and Resistance to Aphid of NTL1 Gene from Narcissus tazetta var.chinensis in Nicotiana alata
摘要
Abstract
The expression of the lectin gene (NTL1) from Narcissus tazzetta var.chinensis in Nicotiana alata was studied by qPCR method in order to determine the aphid-resistant function of NTL1 and cultivate N.alata with aphid-resistant.By means of susceptible to aphid,inoculation in vivo and in vitro,the resistance to aphid in different transgenic tobacco was studied.The result showed that NTL1 gene had been integrated into the tobacco,but the expression of different tobacco was different,the expression of NTL1 in tobacco by descending order was 3,4,1,2.The bioassay of aphid indicated that transgenic tobacco had a certain resistance to aphid,but the capacity of aphid-resistance in different tobacco plant was slightly different;The average density inhibition rate of aphid in inoculation in vivo and in vitro was 8.22%-76.61% and 4.62%-65.65%.The transgenic tobacco could speed up the aphid′s death.The relative expression of NTL1 gene in different transgenic tobacco and the result of resistance to aphid were compared,showed that two results came with a high degree of consistency.关键词
中国水仙/凝集素基因/qPCR/大花烟草/抗蚜性Key words
Narcissus tazetta var.chinensis/Lectin gene/qPCR/Nicotiana alata/Resistance to aphid分类
农业科技引用本文复制引用
杨会苗,陈段芬..中国水仙凝集素基因在大花烟草中的定量表达及抗蚜性分析[J].华北农学报,2017,32(4):78-84,7.基金项目
河北省自然科学基金项目(C2014204131) （C2014204131）
