华北农学报2017，Vol.32Issue(4)：103-106,4.DOI:10.7668/hbnxb.2017.04.017
猪流行性腹泻病毒山西分离株ORF3基因序列分析
Sequence Analysis of the ORF3 Gene of Porcine epidemic diarrhea virus in Shanxi Provine
摘要
Abstract
To investigate the variation the ORF3 gene of Porcine epidemic diarrhea virus,4 PEDV field strains were cloned and sequenced which were isolated from 11 cities of Shanxi from 2014 to 2015.These strains were analyzed and compared with the other published PEDV strains.Sequence analysis of ORF3 gene revealed that the 4 PEDV field strains had several specific nucleotides and amino acids which were different from CV777.The nucleotide and amino acid homologies were 99.6% to 100.0% and 98.7% to 99.6% among 4 PEDV field strains.Compared with CV777,CV777 truncated,attenuated DR13,the nucleotide homologies were 96.6% to 97.0%,90.6%,97.6% to 98.1%,and amino acid homologies were 95.1% to 96.0%,88.0%,97.1% to 98.1%,respectively.Phylogenetic analysis showed that the 4 PEDV field strains were genetically close to other prevalent strains from other parts of China from 2010 to 2015,CH/JL/09 isolated in 2009;However,there were genetically different from CV777,LZC,Brl/87,CV777 truncated,attenuated DR13.关键词
猪流行性腹泻/山西分离株/ORF3基因/序列分析Key words
Porcine epidemic diarrhea/Shanxi strains/ORF3 gene/Sequence analysis分类
农业科技引用本文复制引用
刘文俊,李红丽,赵岳,王娟萍,姚敬明,吴忻,孟帆,韩一超,樊振华,薛翼鹏,米瑞娟..猪流行性腹泻病毒山西分离株ORF3基因序列分析[J].华北农学报,2017,32(4):103-106,4.基金项目
山西省科技攻关项目(20140311020-2-A （）
20150311018-3) （）
