河北医学2017，Vol.23Issue(8)：1236-1241,6.DOI:10.3969/j.issn.1006-6233.2017.08.002
血浆MicroRNA-17-5p和microRNA-20水平与肺动脉高压预后的相关性研究
Correlation between Plasma Levels of MicroRNA-17-5p and microRNA-20 and Prognosis of Pulmonary Arterial Hypertension
张岩 1王聪霞 1李永勤 1张春艳 1胡艳超 1李满祥1
作者信息
- 1. 西安交通大学第二附属医院心血管内科,陕西 西安 710004
- 折叠
摘要
Abstract
Objective:To investigate the correlation between the level of plasma MicroRNA-17-5p and microRNA-20 and the severity and prognosis of pulmonary arterial hypertension(PAH).Methods: A microarray screen was performed on total plasma RNA from 112 patients with PAH and 100 healthy control subjects Quantitative polymerase chain reaction commend increased microRNA-17-5p and microRNA-20a concentrations and was then used to measure microRNA-17-5p and microRNA-20a levels in cohort of patients with PAH's circulating microvesicles and blood cells.Results: Fifty-eight miRNAs showed differences in plasma concentration.MicroRNA-17-5p and microRNA-20a are the largest up-regulation in PAH.Receiver-operator-characteristic analysis showed both raw and normalized plasma microRNA-17-5p and microRNA-20a levels correlated with 2-year survival in patients with PAH.Cox regression analysis confirmed that miRNA-17-5p and miRNA-20a levels were important predictors of PAH survival.Age,baseline cardiac index,World Health Organization functional class,6-minute walk distance,disease duration,and red cell distribution width also predicted survival.Entering these covariates in a multivariable model veried plasma microRNA-17-5p and microRNA-20a levels as independent predictoras of survival in PAH.Conclusion: Increased circulating microRNA-17-5p and microRNA-20a levels are associated with poor survival in PAH.关键词
miRNA-17-5p/miRNA-20a/肺动脉高压Key words
miRNA-17-5p/miRNA-20a/Pulmonary arterial hypertension引用本文复制引用
张岩,王聪霞,李永勤,张春艳,胡艳超,李满祥..血浆MicroRNA-17-5p和microRNA-20水平与肺动脉高压预后的相关性研究[J].河北医学,2017,23(8):1236-1241,6.
