中医学报2017，Vol.32Issue(8)：1535-1539,5.DOI:10.16368/j.issn.1674-8999.2017.08.404
补肾益髓法对地中海贫血患者β-珠蛋白及BCL11A基因表达的影响
Influence on the Beta Globin and BCL11A Gene Expression by Method of Invigorating Kidney and Benefiting Marrow in Patients with Mediterranean Anemia
摘要
Abstract
Objective:To investigate the effect of the Beta globin regulation effect and BCL11A gene expression by method of invigorating kidney and benefiting marrow in patients with Mediterranean anemia.Methods:82 patients were selected and randomly divided into two groups.41 cases in the control group were treated by clinical routine treatment,41 cases in the experiment group were treated on the base of the control group with method of invigorating kidney and benefiting marrow.Composition:Fructus Corni,Radix Polygoni Multiflori,Radix Rehmanniae Preparata,Fructus Psoraleae,Astragalus,Turtle Shell,Licorice.Each bag is 10 g,equivalent to 2.27 g crude drug Chinese medicinc raw material,by Daxing preparation center of Guang'anmen Hospital of China Academy of Chinese Medical Science,1 bag per day,3 times a day,warm boiling water,for 3 months.The change of blood routine,β-mRNA,γ-mRNA,BCL11A levels and clinical efficacy in two groups before and after treatment were observed.Results:Compared with before treatment,two groups of patients with hemoglobin (Hb),red blood cell (RBC),reticulocyte (Ret),erythrocyte mean corpuscular(MCV),γ-mRNA and γ/(γ+ β) mRNA levels were significantly higher than before treatment (P ＜ 0.05),BCL11A and TCM symptom scores were significantly lower than before treatment(P ＜ 0.05).Comnpared with the control group,the experimental group of patients after treatment of Hb,RBC,Ret,MCV,γ-mRNA,γ/(γ + β) mRNA and effective rate of treatment was higher than the control group,the level of BCL11A and symptom scores were lower than the control group;the differences were statistically significant (P ＜ 0.05).There was no significant difference in the level of β-mRNA between the two groups before and after treatment (P ＞ 0.05).Conclusion:Method of invigorating kidney and benefiting marrow can effectively reduce the expression level of BCL11A in patients with thalassemia,,and improve Hb,WBC,Ret and γ-mRNA gene expression level,and has better clinical efficacy,good safety,but has no effect on the beta mRNA level,and the specific mechanism needs be further studied.关键词
地中海贫血/补肾益髓法/β-珠蛋白基因/B细胞慢性淋巴细胞性白血病/淋巴瘤11A/γ珠蛋白基因Key words
mediterranean anemia/method of invigorating kidney and benefiting marrow/beta globin gene/B cell chronic lymphocytic leukemia / lymphoma 11A/gamma globin gene分类
医药卫生引用本文复制引用
李娜..补肾益髓法对地中海贫血患者β-珠蛋白及BCL11A基因表达的影响[J].中医学报,2017,32(8):1535-1539,5.基金项目
北京市科技计划项目(Z090507017709007) （Z090507017709007）
