河南农业科学2017，Vol.46Issue(9)：79-82,87,5.DOI:10.15933/j.cnki.1004-3268.2017.09.014
西花蓟马取食二斑叶螨危害的番茄植株后体内酶活性的变化
Variance in Enzymes Activities of Frankliniella occidentalis(Pergande) Feeding on Tomato Plant Damaged by Tetranychus urticae Koch
摘要
Abstract
In order to explore the effect of induced defensive responses of tomato plant damaged by Tetranychus urticae on Frankliniella occidentalis,the influences of tomato plant damaged by T.urticae at different densities(5 mites/leaf,15 mites/leaf) and different time (3 d,5 d,7 d) on enzymes activities of F.occidentalis were detected.The results showed that when F.occidentalis fed on tomato plant damaged by T.urticae,the activities of protective enzymes (POD,CAT and SOD) and defensive enzymes (CarE,GSTs and MFO) in thrips were different from those in thrips feeding on healthy tomato plant.The activities of SOD in thrips were inhibited no matter which density and damage time of T.urticae.When thrips fed on tomato leaf damaged by T.urticae at the density of 5 mites/ leaf and 15 mites/leaf for three periods,the activities of SOD were reduced by 15.8％,27.7％,14.4％,and 26.4％,8.8％,17.3％ respectively.The enzyme activities of POD,CAT,CarE,GSTs and MFO were highest in thrips feeding on tomato plant damaged by T.urticae at the density of 5 mites/leaf for 3 days,and increased by 126.4％,17.7％,7.2％,6.7％ and 17.1％ respectively.The activities of the five enzymes all showed a downward trend with density increase and time extension,while the changes of protective and defensive enzymes were different.These results indicated that damage of T.urticae could induce the defensive responses of tomato plant,and cause the significant changes of protective and defensive enzymes activities of F.occidentalis.关键词
二斑叶螨/番茄植株/西花蓟马/保护酶/解毒酶Key words
Tetranychus urticae Koch/tomato plant/Frankliniella occidentalis (Pergande)/protective enzymes/defensive enzymes分类
农业科技引用本文复制引用
温娟,郅军锐,曾广..西花蓟马取食二斑叶螨危害的番茄植株后体内酶活性的变化[J].河南农业科学,2017,46(9):79-82,87,5.基金项目
国家自然科学基金项目(31660516) （31660516）
贵州省普通高等学校系统与应用蜱螨学创新团队项目(黔教合人才团队字[2014]33) （黔教合人才团队字[2014]33）
贵州省国际科技合作基地项目(黔科合平台人才[2016]5802) （黔科合平台人才[2016]5802）
