河南农业科学2017，Vol.46Issue(9)：88-91,97,5.DOI:10.15933/j.cnki.1004-3268.2017.09.016
马拉硫磷降解菌株MN-13的降解性能测定及其培养基优化
Degradation Property Characterization and Culture Medium Optimization of Malathion-Degrading Strain MN-13
摘要
Abstract
In order to solve the serious non-point source soil pollution problem caused by pesticides and use microbial strains to degrade organophosphorus residues in soil,molybdenum blue spectrophotometry was used to test the performance of strain MN-13 to degrade malathion and other organophosphorus pesticides.At the same time,the methods of single factor selection and orthogonal optimization were applied to optimize the medium.The experimental results showed that the degradation rates of strain MN-13 on 25％ malathion,40％ omethoate and 50％ ethyl parathion were 91.52％,69.24％ and 49.68％ respectively.By single factor experiments,carbon source,nitrogen source,NaCl and pH of medium were preliminarily determined as glucose 1.5％,beef extract 1.0％,NaCl 0.5％ and pH 8.0.After the orthogonal experiment,the medium composition was optimized as follows:glucose 1.5％,beef extract 1.2％,NaCl 0.6％ and pH 8.5.The OD600 value of this strain was increased by 33.1％ after optimization.From the above results,it was concluded that the strain MN-13 could effectively degrade malathion and other organophosphorus pesticides,and it grew better after medium optimization.关键词
马拉硫磷/微生物降解/MN-13菌株/降解率/培养基/优化Key words
malathion/microbial degradation/MN-13/degradation rate/medium/optimization分类
资源环境引用本文复制引用
高小朋,包本开,杨佳娟,刘蕊..马拉硫磷降解菌株MN-13的降解性能测定及其培养基优化[J].河南农业科学,2017,46(9):88-91,97,5.基金项目
国家大学生创新创业训练计划项目(201510719287) （201510719287）
陕西省大学生创新创业训练计划项目(1447) （1447）
