河南农业科学2017，Vol.46Issue(9)：114-117,4.DOI:10.15933/j.cnki.1004-3268.2017.09.021
紫叶加拿大紫荆试管苗两步生根培养技术研究
Study on Two-step Rooting Culture of Cercis canadensis 'Forest Pansy ' Seedlings in Vitro
摘要
Abstract
To solve the rooting problem of Cercis canadensis ''Forest Pansy'' seedlings in vitro,and improve rooting rate and the quality of its roots,we used test-tube seedlings of Cercis canadensis ''Forest Pansy'' as experimental material and adopted a two-step rooting method to study the effects of induction time,concentration of IBA and NAA,seeding height and transopenerational cycle on the rooting.The results indicated that the best induction root was achieved by the two-step rooting method:firstly the plantlets in vitro cultured for 30 d and more than 4 cm were incubated on 1/2MS medium with 5 mg/L IBA and 0.5 mg/L NAA in the dark for 11 days,and then the shoots were transferred to the MS medium without plant growth hormone in the normal light conditions for 30 d.As a result,95.4％ of the rooting rate was obtained under above conditions,and the rooting number was 7.71,rooting length was 3.56 cm.关键词
紫叶加拿大紫荆/离体培养/两步生根法Key words
Cercis canadensis 'Forest Pansy '/in vitro culture/a two-step rooting method分类
农业科技引用本文复制引用
李艳敏,王利民,王慧娟,孟月娥,张和臣,董晓宇,符真珠..紫叶加拿大紫荆试管苗两步生根培养技术研究[J].河南农业科学,2017,46(9):114-117,4.基金项目
河南省科技攻关项目(162102110103) （162102110103）
河南省财政预算项目(豫财预[2016]78号) （豫财预[2016]78号）
