河南农业科学2017，Vol.46Issue(9)：144-148,5.DOI:10.15933/j.cnki.1004-3268.2017.09.027
河南地区猪呼吸道隐性感染大肠杆菌耐药基因检测及分析
Detection and Analysis of Escherichia coli Resistance Gene inRecessive Infection of Pigs in Henan Province
摘要
Abstract
In order to understand the distribution of the resistance gene and drug resistance to β-lactam antibiotics,Tetracycline antibiotics and Florfenicol of the pig silent infection pathogenic bacteria in the respiratory tract in Henan province,the mircrobroth dilution method was adopted to analyze the drug resistance phenotypes and the drug susceptibility to 16 kinds of drugs such as Penicillin,Cefepime,Gentamycin,Doxycycline and Florfenicol of 31 stains of Escherichia coli isolated from the pig lungs in Henan province,and the PCR was adopted to analyze the drug resistance genes to β-lactam antibiotics,Tetracycline antibiotics and Florfenicol of 31 strains of Escherichia coli.The results showed that the drug resistance rates of the tested bacteria to Penicillin,Ampicillin and Amoxicillin were 100％,the drug resistance rates of the tested bacteria to Ceftiofur Cefotaxime and Cefepime respectively were 29.03％,32.26％ and 3.23％,the drug resistance rates of the tested bacteria to Tetracycline and Doxycycline respectively were 83.87％ and 70.97％,and the drug resistance rates of the tested bacteria to Florfenicol was 48.39％.The tested bacteria were more sensitive to Quinolones,such as Ciprofloxacin and Enrofloxacin,and Aminoglycosides,such as Gentamycin and Amikacin.The detection rate of β-lactam antibiotics resistance gene such as TEM,SHV,CTX-M-U,CTX-M-1 and CTX-M-9 respectively were 54.84％,61.29％,25.81％,9.68％ and 9.68％,the detection rate of Tetracycline antibiotics resistance gene such as tet(A),tet(M) and tet(L) were respectively 87.10％,54.84％ and 19.35％,the positive bacteria of tet? and tet(O) are all detected one strain,tet(B),tet(K) and tet(W) were not detected,and the detection rate of Florfenicol resistance gene(floR) was 54.84％.Two strains of bacteria had seven resistance genes and 19 strains had four or more resistant genes.It can be seen that multiple drug resistance genes co-mediated the multidrug resistance of Escherichia coli had become a popular trend.关键词
猪/呼吸道/隐性感染/大肠杆菌/耐药基因Key words
porcine/respiratory tract/recessive infection/Escherichia coli/resistance gene分类
农业科技引用本文复制引用
李进福,丁海峰,李小申,刘保光,苏嘉,苑丽..河南地区猪呼吸道隐性感染大肠杆菌耐药基因检测及分析[J].河南农业科学,2017,46(9):144-148,5.基金项目
国家自然科学基金项目(31201965) （31201965）
