核农学报2017，Vol.31Issue(10)：2023-2031,9.DOI:10.11869/j.issn.100-8551.2017.10.2023
打叶留顶对烤烟上部叶和根系养分、烟碱及其合成酶的影响
Effects of Keeping Topping and Threshing on Nutrient, Nicotine and Enzyme Activity of Upper Leaves and Root of Flue-cured Tobacco
摘要
Abstract
To explore the effects of topping-control technology on nicotine content in upper leaves of flue-cured tobacco and its physiological indexes,improve the industrial availability of upper leaves,the dynamic changes of nitrogen,potassium and nicotine content and physiological indexes such as synthetase activity and endogenous hormone in upper leaves and root system in box plot by topping-regulation were studied in this paper.The results showed that dry matter accumulation in upper leaves and root follow the order of topping ＞ keeping stem unremoved ＞ keeping flower unremoved,and tobacco by topping and keeping stem unremoved were higher than that of keeping flower unremoved by 32.55％ and 5.99％ at 40 d.The contents of Nitrogen and potassium in upper leaves by keeping flower unremoved were the highest,but the accumulation of nitrogen and potassium in upper leaves by topping was the highest.Nitrogen and potassium accumulation in upper leaves by topping were higher than that by keeping flower unremoved by 39.73％ and 40.35％ at 40d.The nicotine content and accumulation in upper leaves and roots followed the order of topping ＞ keeping stem unremoved ＞ keeping flower unremoved.Nicotine accumulation of keeping flower and stem unremoved were decreased by 53.72％ and 35.82％ compared with the topping at 40d.The increase degree of root volume of keeping flower unremoved was low,root activity,ornithine decarboxylase (ODC),putrescine-N methyltransferase (PMT) and N-methyl putrescine oxidase (MPO) activity remained unchanged overall,and the content of auxin (IAA) changed a little;while root volume,root activity,ODC,PMT,MPO activity and IAA content of topping and keeping stem unremoved treatments significantly enhanced than those of keeping flower unremoved.Root activity,ODC and IAA content of topping were increased by 36.68％,119.61％ and 74.19％,respectively,compared with keeping flower unremoved at 10d.Root nicotine synthesis and root activity,the content of IAA and the activity of nicotine synthetase were positively correlated.Therefore,the technique of keeping topping and threshing affected the uptake of nitrogen and potassium and nicotine content in the upper leaves by adjusting the activity of nicotine synthetase,IAA content in the root,and weaken the capacity of root second growth and nicotine synthesis.The research lay in the improvement of the internal quality of upper leaves of flue-cured tobacco and improved its industrial availability.关键词
打叶留顶/烤烟/养分/烟碱/合成酶Key words
keeping topping and threshing/flue cured tobacco/nutrient/nicotine/synthetase引用本文复制引用
罗斐,陆新莉,李朝阳,武丽,李章海,刘雪梅,张庆珠,唐兴贵..打叶留顶对烤烟上部叶和根系养分、烟碱及其合成酶的影响[J].核农学报,2017,31(10):2023-2031,9.基金项目
贵州省黔南州科技局重点科技项目(黔南科合农[2013]34) （黔南科合农[2013]34）
