河南中医2017，Vol.37Issue(8)：1373-1375,3.DOI:10.16367/j.issn.1003-5028.2017.08.0483
水蛭酶解前后对缺血性脑卒中大鼠治疗作用比较
Comparative Study of the Therapeutic Effects of Hirudo Before and After Enzymolysis on Ischemic Stroke Rats
摘要
Abstract
Objective:To prepare hirudin by using enzyme hydrolysis method which will act on rats with stroke in parallel,and compare the efficacies before and after enzymatic hydrolysis.Methods:All the rats were implanted with electrocoagulation of middle cerebral artery;80 rats were anesthetized with chloral hydrate 0.35 g · kg-1 (10％,3.5 mL · kg-1);20 rats,chosen into the sham operated group,were only cut in the skin and then sutured and fed.The rats'behaviors were scored after cerebral ischemia for 24 hours.The ones who ≥ 1 score after modeling were chosen and randomly divided into 3 groups:the model group (sterile distilled water),the leech group 0.8 g · kg-1,and the hirudin group 0.64 g · kg-1,with 20 rats in each group.They received intragastric administration,once a day,for 7 days continuously,and the volume of administration was 1.0 mL/100 g body mass.And 24 hours after the last administration,various indexes were checked.The rats in each group were injected with chloral hydrate 0.35 g · kg-1 (10％,3.5 mL · kg-1);femoral artery blood 2ml was taken,3 500 circles each minute,10 minutes after being centrifuged,serum was taken,and SOD and MDA were measured.The brain was taken (removing the olfactory bulb,cerebellum and lower brainstem),frozen for 10 minutes with-20 degrees,and then cut into the coronal slices with a thickness of about 2mm(5 or 6 slices),immediately placed in 1％ TTC solution,incubated for 40 minutes with 37 ℃.The ischemic areas were white and the non ischemic areas were red.The ischemic areas and non ischemic areas were separated according to coloration,and weighed respectively,and the percentage of brain weight in the ischemic zone (％) was calculated.Results:After 7 days'treatment,compared with the sham operation group,the rats in the model control were circling to the right,toppling to the opposite side when walking and other symptoms of neurological damage,and their behavior scores were significantly higher than those of the sham operation group (P ＜0.01).Compared with the model control group,the neurobehavioral score of the leech peptide group and the leech group were significantly reduced (P ＜0.01,P ＜0.05).Conclusion:The hirudin peptide obtained from hirudo by the enzymatic hydrolysis is superior to leech in the treatment of ischemic stroke rats.关键词
水蛭/酶解/水蛭肽/缺血性脑卒中/大鼠Key words
leech/enzymolysis/hirudin/ischemic stroke/rats分类
医药卫生引用本文复制引用
陈翠华,蒋艳玲..水蛭酶解前后对缺血性脑卒中大鼠治疗作用比较[J].河南中医,2017,37(8):1373-1375,3.基金项目
河南省重点科技攻关项目(编号:102102310089) （编号:102102310089）
