家畜生态学报2017，Vol.38Issue(8)：29-34,6.
梯度添加硝酸盐或硫酸盐对瘤胃体外发酵和总菌数量的影响
Effects of Gradient Addition of Nitrate and Sulfate on Rumen Fermentationand Total Bacteria Count in vitro
摘要
Abstract
The present study focused on effects of gradient addition of nitrate or sulfate on rumen fermentation and the total bacteria count by batch fermentation with the method of in vivo gas production, aiming to investigate gradient addition of nitrate or sulfate on the adaptation of rumen microbes.The fluid was collected from three fistulated Xinong Saanan dairy goats.Experiment design contained addition of sodium nitrate, potassium slufate and the control with neither of them.The treatment was added 2-5 mM nitrate or sulfate in 5 days by grads.Total gas, pH, volatile fatty acids (VFA), ammonia and the number of total bacteria count were measured.Results showed: (1) The volumes of the total gas were significantly lower with addition of 2~5 mM nitrate in 3 to 5 days (P<0.05) and addition of slufate on the first day, whereas, on other days, inclusion of nitrate or sulfate had no difference on the volumes of the total gas (P>0.05).(2) In a way, addition of 2~5 mM nitrate or sulfate changed the concentration and proportion of VFAs, but had no difference on TVFA (P>0.05);with the increasing of nitrate, the ratio of acetate to propionate decreased gradually.(3) Addition of 2 mM nitrate had a trend of ammonia increasing (P<0.1) and 3~5 mM increased ammonia significantly in 2 to 5 days (P<0.05);In the first four days, inclusion of 2~5 mM slufate did not change ammonia compared with the control, but increased ammonia significantly on the last day (P<0.05);(4) Addition of 2~5 mM nitrate or sulfate had no significant effect on the number of total bacteria (P>0.05).Therefore, it is concluded that gradient addition of nitrate or sulfate had no significant inhibitory effect on the fermentation parameters and total bacteria count in batch cultures.关键词
硝酸盐/硫酸盐/体外产气法/瘤胃发酵Key words
nitrate/slufate/in vivo gas production/rumen fermentation分类
农业科技引用本文复制引用
刘丽慧,刘宝龙,崔宏晓,孙菲菲,谢光中,徐秀容..梯度添加硝酸盐或硫酸盐对瘤胃体外发酵和总菌数量的影响[J].家畜生态学报,2017,38(8):29-34,6.基金项目
西北农林科技大学基本科研业务费(QN2452015147) （QN2452015147）
