基础医学与临床2017，Vol.37Issue(9)：1286-1291,6.
CNTN-1促进人食管癌EC9706细胞侵袭和迁移
CNTN-1 promotes the invasion andmigration of human esophageal cancer EC9706 cell line
曹燕飞 1任睿 2罗向晖 1杨晔 1王水利3
作者信息
- 1. 陕西省人民医院 胸外科,陕西 西安 710068
- 2. 陕西省新安中心医院 妇产科,陕西 西安 710048
- 3. 陕西省人民医院 呼吸内科,陕西 西安 710068
- 折叠
摘要
Abstract
Objective To investigate the effects of CNTN-1 on the invasion and migration of human esophageal cancer EC9706 cells and the possible mechanism.Methods The expression of CNTN-1 in human esophageal cancer EC9706 cells was measured by qPCR and Western blot.After transfection with CNTN-1 siRNA or CNTN-1, the cells were divided into control group, scrambled siRNA group, CNTN-1 siRNA group, pcDNA3.1-vector group and pcDNA3.1-CNTN-1 group.Cell proliferation, invasion and migration were respectively analyzed by BrdU assay and Transwell test.The expression of MMP-2 and MMP-9 were detected by qPCR and Western blot.Results The mRNA and protein expression of CNTN-1 were significantly upregulated in EC9706 cells.Compared with control, cell proliferation, invasion and migration, as well as the expression of MMP-2 and MMP-9 were significantly decreased by CNTN-1 siRNA, while they were increased by CNTN-1 overexpression (P<0.05).ConclusionsCNTN-1 can influence the invasion and metastasis of esophageal cancer cells through the regulation of the expression of MMP-2 and MMP-9.关键词
食管癌/CNTN-1/侵袭/迁移Key words
esophageal cancer/CNTN-1/invasion/migration分类
医药卫生引用本文复制引用
曹燕飞,任睿,罗向晖,杨晔,王水利..CNTN-1促进人食管癌EC9706细胞侵袭和迁移[J].基础医学与临床,2017,37(9):1286-1291,6.
