激光生物学报2017，Vol.26Issue(4)：350-359,10.DOI:10.3969/j.issn.1007-7146.2017.04.011
全基因组序列的系统发育与重组分析鉴定我国马铃薯Y病毒株系
Determination of Potato virus Y Strain in China Based on Phylogenetic and Recombination Analyses of the Complete Genomic Sequences
摘要
Abstract
To investigate the composition and distribution of Potato virus Y (PVY) strains across different hosts in China, phylogenetic and recombination analyses were studied using 31 complete coding sequences in PVY isolates.Phylogenetic analysis revealed that the NJ and ML bootstrap trees had broadly similar topologies, in which 6 isolates of GF_YL20, FZ10, DF, SD1, 1116 and 9703-3 are divergent from all known PVY strains and the remaining isolates from the same strain tended to group together with high bootstrap supports, showing a distinct strain feature among PVY isolates.Significant recombination signals were identified in 28 out of the 31 PVY isolates and the majority of them classified to PVYN-Wi, PVYNTN and PVYNTN-NW.Further analysis showed that 5 isolates of 1104, DF, SD1, 1116 and 9703-3 are novel N×O recombinants.Results from the composition and distribution of PVY strains indicated that recombinant strains were dominant in the PYV isolates in China.Thus, it is important to monitor the development of these recombinants in practice.The above results showed that all PVY isolates could be distinguished accurately using phylogenetic combined with recombination analyses.关键词
马铃薯Y病毒/系统发育分析/重组分析/株系鉴定Key words
Potato virus Y/phylogenetic analysis/recombination analysis/strain determination分类
农业科技引用本文复制引用
蔡伟,杨宏凯,沈建国,邹文超,高芳銮..全基因组序列的系统发育与重组分析鉴定我国马铃薯Y病毒株系[J].激光生物学报,2017,26(4):350-359,10.基金项目
国家重点研发计划项目(2016YFF0203203) （2016YFF0203203）
福建检验检疫局科技计划项目(FK2015-FQ0001) （FK2015-FQ0001）
福清市科技计划项目(FQ201501) （FQ201501）
