实用临床医药杂志2017，Vol.21Issue(15)：74-77,4.DOI:10.7619/jcmp.201715020
肾细胞癌中Wif-1基因的甲基化状态及表达
Methylation and mRNA expression of Wif-1 gene in renal cell carcinoma
倪晓辰 1张爱莉 1卫书飞1
作者信息
- 1. 河北医科大学第四医院 泌尿外科, 河北 石家庄, 050011
- 折叠
摘要
Abstract
Objective To detect the methylation of Wif-1 gene promoter and its mRNA expression level in renal cell carcinoma, and to explore its possible mechanisms in the development of renal cell carcinoma.Methods The methylation-specific polymerase chain reaction (MSP) and reverse transcription-polymerase chain reaction (RT-PCR) were applied to investigate the methylation status of Wif-1 gene promoter and its mRNA expression levels in renal cell carcinoma (RCC) and normal kidney tissue.Results The percentage of methylation of Wif-1 gene in RCC tissue was significantly higher than that in the corresponding normal kidney tissue (P<0.05).The relative expression quantity of Wif-1 mRNA in RCC tissue was significantly lower than that in corresponding normal kidney tissue (P<0.05).In the RCC tissue, the relative expression of mRNA in the Wif-1 methylation positive group showed no significant difference with that in the methylation negative group.Conclusion Hypermethylation of the Wif-1 promtor in RCC is a frequent phenomenon.关键词
肾细胞癌/Wif-1/甲基化/表达Key words
renal cell carcinoma/Wnt inhibitory factor-1/methylation/expression分类
医药卫生引用本文复制引用
倪晓辰,张爱莉,卫书飞..肾细胞癌中Wif-1基因的甲基化状态及表达[J].实用临床医药杂志,2017,21(15):74-77,4.
