解放军医学院学报2017，Vol.38Issue(8)：793-797,5.DOI:10.3969/j.issn.2095-5227.2017.08.021
低剂量地西他滨对巨噬细胞极化的调节作用
Effect of low dose decitabine on macrophage polarization
高杰清 1张琪 2尹雅琪 2母义明 2郝好杰 2彭彦平3
作者信息
- 1. 首都医科大学附属北京康复医院 内分泌科,北京 100144
- 2. 解放军总医院 内分泌科,北京 100853
- 3. 解放军总医院 基础医学所,北京 100853
- 折叠
摘要
Abstract
Objective To observe the effect of decitabine (DAC) on macrophage polarization.Methods Bone marrow-derived macrophages (BMDMs) were obtained from six week-old C57/6L mice.The cell viability was detected by CCK8,western blot technique was adopted to detect macrophage phenotype changes treated by DAC.Macrophages were divided into control group,LPS group (LPS induced) and DAC group (LPS+DAC treatment),the levels of inflammatory factors were detected by qRT-PCR.The expression of macrophage subtype maker proteins was detected by western blot.Results Decitabine did not affect the activity and proliferation of macrophages.Compared with LPS group,DAC obviously depressed the expression of pro-inflammatory factors with IL-6 (99％) and CXCL10 (98％) decreasing most,and it upregulated anti-inflammatory factors with IL-4 increasing most by about 3 times (P ＜ 0.05).Western blot results showed that DAC also obviously decreased M1 macrophage surface maker iNOS by 72％ (1.4 vs 0.4,P=-0.001) and upregulated M2 macrophage surface maker Arg-1 for 1.8 times with significant differences (0.5 vs 0.9,P=0.01).Conclusion Decitabine treatment can promote the polarization of macrophage to regulatory macrophage (M2) and restrain chronic inflammation.关键词
地西他滨/巨噬细胞极化/炎症Key words
decitabine/macrophage polarization/inflammation分类
医药卫生引用本文复制引用
高杰清,张琪,尹雅琪,母义明,郝好杰,彭彦平..低剂量地西他滨对巨噬细胞极化的调节作用[J].解放军医学院学报,2017,38(8):793-797,5.
