摘要
Abstract
Objective To investigate the effect of arsenic trioxide (As2O3) on malignant pleural effusion (MPE) in mice model caused by lung cancer.Methods Eighty-four MPE mice models were established firstly.Random allocation was taken to divide them into three groups:experimental group,negative control and positive control.As2O3 was intraperitoneally injected into mice of experimental group,normal saline and bleomycin were administered to negative control and positive control respectively,with the same dose for a week.After administration,MPE volume was measured,expressions of VEGF and VEGF mRNA in MPE were detected by ELISA and RT-PCR,overall survival of MPE mice was analyzed by Kaplan-Meier.Results After administration of As2O3,MPE volume was attenuated significantly in experimental group when compared with negative control [(0.43 ± 0.11) ml vs (0.92 ± 0.53) ml,P=0.000],and positive control [(0.43 ± 0.11) ml vs (0.63 ± 0.32) ml,P=0.001],and the expression of VEGF in MPE of As2O3 group was remarkably lower than those of negative control [(493.07 ± 35.10)pg/ml vs (2500.30 ± 210.32)pg/ml,P=0.001] and positive control [(493.07 ± 35.10) pg/ml vs (671.23 ± 34.21) pg/ml,P=0.003].Compared with negative and positive controls,VEGF mRNA expression level in MPE decreased significantly after intervened with As2O3 [(0.32 ± 0.02) vs (1.02 ± 0.32),P=0.001;(0.32 ± 0.02) vs (0.53 ± 0.04),P=-0.004].The survival time of mice in As2O3 group was (21.20 ± 2.86) d,which was significantly longer than those of negative control [(13.05 ± 3.50) d,P=0.031] and positive control [(17.60 ± 3.22) d,P=0.013].Conclusion The results suggest that As2O3 inhibits MPE promotion and lengthen mice' survival time by reducing transcription and expression of VEGF.This study provides basis by animal experiment for MPE treatment.关键词
三氧化二砷/恶性胸腔积液/生存时间/小鼠Key words
arsenic trioxide/malignant pleural effusion/survival time/mice分类
医药卫生
