检验医学与临床2017，Vol.14Issue(17)：2523-2525,3.DOI:10.3969/j.issn.1672-9455.2017.17.010
MALDI-TOF质谱仪进行临床酵母菌鉴定的方法学评价
Methodological evaluation of MALDI-TOF MS for conducting identification of clinically isolated yeast
李艳君 1董蓉 1赵强元1
作者信息
- 1. 中国人民解放军海军总医院检验科,北京 100048
- 折叠
摘要
Abstract
Objective To probe into the superiority of MALDI-TOF MS in the identification of clinical yeast to provide the basis for early diagnosis and treatment of clinical fungal infection.Methods A total of 206 strains of yeast clinically isolated in our hospital from August 2015 to March 2016 were collected and identified with MALDI-TOF mass spectrometer,VITEK2 compact microbial analyzer and chromogenic culture respectively.The ITS sequencing results were used as the final confirmation one.The accuracies of identifying yeast were compared among these 3 kinds of method.Results The accuracy rates of 3 kinds of method for identifying yeast were 82.5% for VITEK2 Compact,67.0% for chromogenic method and 92.7% for MALDI-TOF mass spectrometry.The difference between MALDI-TOF and VITEK2 compact was statistically significant(P=0.006),and the difference between MALDI-TOF and chromogenic method was statistically significant(P=0.001).Conclusion Compared with the VITEK2 Compact microbial analyzer and the color developing method,the MALDI-TOF mass spectrometer has the advantages of rapidness,simple operation,accurate results and so on.关键词
MALDI-TOF质谱仪/酵母菌/内转录间区测序Key words
MALDI-TOF MS/yeast/ITS sequencing引用本文复制引用
李艳君,董蓉,赵强元..MALDI-TOF质谱仪进行临床酵母菌鉴定的方法学评价[J].检验医学与临床,2017,14(17):2523-2525,3.
