临床口腔医学杂志2017，Vol.33Issue(9)：515-519,5.DOI:10.3969/j.issn.1003-1634.2017.09.002
RNA干扰沉默TGF-β1基因对口腔鳞癌细胞CAL-27生物学行为的影响
Effects of TGF-β1 genesliencing by RNAi on the biological behavior of oral squamous cell carcinoma CAL-27 cells in vitro
摘要
Abstract
Objective:To investigate the effect of RNA interference(RNAi)targeting transforming growth factor-β1(TGF-β1)gene on the invasion and migration of oral squamous cell carcinoma(OSCC)cellline CAL-27.Methods:The oral squamous cell carcinoma CAL-27 cells cultured in vitro were randomly divided into the observation group,negative control group.CAL-27 cells in the observation group and negative control group were transfected with TGF-β1-siRNA and Negative control siRNA(NC-siRNA)in vitro by using Lipojet.The Real time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression of TGF-β1 at 24 h after transfection.The distribution of cell cycle phases was determined by flow cytometry.Plate cloning assay and Cell counting kit-8(CCK-8)assay was used to observe the proliferation of the cells after transfection.Transwell assay were used to observe the migration and invasion abilities of the cells after transfection.Western blotting was used to explore the expression of protein at 24 h after transfection.Results:At 24 h after transfection,compared with the negative control group,the expression of TGF-β1 and β-catenin protein was significantly lower,the number of penetrating cells was significantly less.A450 in the observation group was lower than that in the negative control group at 48 and 72 h after transfection.Conclusion:RNA interference targeting TGF-β1 gene could suppress proliferation,invasion and migration of oral squamous cell carcinoma cells effectively,and reverse the malignant phenotypes of oral squamous cell carcinoma cells to some extent.关键词
TGF-β1/口腔鳞状细胞癌/小干扰RNA/增殖/迁移Key words
TGF-β1/Oral squamous cell carcinoma/siRNA/Proliferation/Migration分类
生物科学引用本文复制引用
陈婉丽,铁朝荣..RNA干扰沉默TGF-β1基因对口腔鳞癌细胞CAL-27生物学行为的影响[J].临床口腔医学杂志,2017,33(9):515-519,5.基金项目
湖北省自然科学基金(220100321) （220100321）
