临床神经病学杂志2017，Vol.30Issue(4)：285-289,5.
褐藻多糖硫酸酯对1-甲基4-苯基吡啶离子损伤的MN9D细胞内氧化应激及组织蛋白酶D-单克隆凋亡相关蛋白的作用
Effect of fucoidan on introcelleral oxidative stress and Cathepsin D-Apoptosis associated protein Bax of the 1-methyl-4-phenylpyridinium intoxicated MN9D cell model
摘要
Abstract
Objective To explore the effect of fucoidan(FUC) introcelleral oxidative stress and Cathepsin D-Apoptosis(CatD) associated protein Bax of the 1-methyl-4-phenylpyridinium(MPP+) intoxicated MN9D cell model.Methods PD cell model was established by MN9D cells model which damaged by 100 mol/L MPP+.The cell viability of PD cell model was observed after FUC pretreatment.The inhibitory rate of superoxide dismutase(SOD) and glutathione reductase (GSH) were measured by fluorescence detection.The expression of Bax, CatD and microtubule-associated protein light chain3(LC3-Ⅱ) were measured by western blot.Results Compared with MPP+ group, the cell viability were significantly increased than those after 1×10-6, 10-5, 10-4 mol/L FUC pretreatment(all P<0.01).Compared with control group, the inhibitory rate of SOD and GSH in MPP+ group were significantly decreased(all P<0.05).Compared with MPP+ group, the inhibitory rate of SOD and GSH in FUC pretreatment group and SEL pretreatment group were significantly higher(all P<0.01).There was no significant difference in inhibitory rate of SOD and GSH between FUC pretreatment group and SEL pretreatment group.The expression of CatD, LC3-Ⅱ and Bax protein in MPP+ group were significantly increased than those in control group(all P<0.001).The expression of CatD, LC3-Ⅱ and Bax protein in FUC pretreatment group, SEL pretreatment group and CatD inhibitor group were significantly decreased than those in MPP+ group(all P<0.001).There was no significant difference of expression of CatD, LC3-Ⅱand Bax protein between FUC pretreatment group, SEL pretreatment group and CatD inhibitor group.Conclusions FUC has protective effect on PD cell model.The mechanism are protecting cell lysosomes, inhibiting expression of CatD-Bax, reducing oxidative stress and inhibiting apoptosis.关键词
褐藻多糖硫酸酯/MN9D细胞/1-甲基4-苯基吡啶离子/氧化应激/细胞凋亡Key words
fucoidan/MN9D cells/1-methyl-4-phenylpyridinium/oxidation stress/apoptosis分类
医药卫生引用本文复制引用
梁志刚,孙旭文,李敏,窦连伟,陶曼丽,王晓民..褐藻多糖硫酸酯对1-甲基4-苯基吡啶离子损伤的MN9D细胞内氧化应激及组织蛋白酶D-单克隆凋亡相关蛋白的作用[J].临床神经病学杂志,2017,30(4):285-289,5.基金项目
烟台市科技发展项目资助(2012077) （2012077）
