环境与职业医学2017，Vol.34Issue(8)：707-711,717,6.DOI:10.13213/j.cnki.jeom.2017.17123
氯化锰致人骨髓神经母细胞瘤细胞株线粒体损伤及对多巴胺分泌和PARK2表达的影响
Effects of manganese chloride on mitochondrial damage, dopamine secretion, and expression of PARK2 in human bone marrow neuroblastoma cells
摘要
Abstract
[Objective] To study the effects of manganese chloride (MnC12) on mitochondrial damage,oxidative stress,secretion of dopamine,and expression of PARK2 in human bone marrow neuroblastoma cells (SH-SY5Y).[Methods] SH-SY5Y cells were exposed to MnC12 (0,100,300,and 500 μmol/L) for 24 h.MTT assay was used to measure inhibition rate of the cells (mitochondrial damage),graphite furnace atomic absorption spectrometry for intracellular manganese concentration,water soluble tetrazolium salt (WST-1) assay for superoxide dismutase (SOD) activity,thiobarbituric acid assay for malondialdehyde (MDA) level,reverse phase high performance liquid chromatography-fluorometry for dopamine (DA) level,RT-PCR for the expression of PARK2 mRNA,and Western blot for the expression of Parkin protein,respectively.[Results] The cell inhibition rates (mitochondrial damage) of the 300 and 500 μmol/L MnCl2 groups were increased compared with the control group (P ＜ 0.01).The intracellular manganese concentration of the SH-SY5Y cells was increased in the MnC12 groups compared with the control group (P＜ 0.05 or P ＜ 0.01).Compared with the control group,decreasing SOD activities and DA levels and increasing MDA levels were observed in the 300 and 500 μmol/L MnC12 groups (P＜ 0.01).At the same time,the expressions of PARK2 mRNA and Parkin protein were also decreased (P＜ 0.01).The results of correlation analysis revealed that the expression of PARK2 mRNA was inversely correlated with cell inhibition rate (mitochondrial damage) (r=-0.872),intracellular manganese concentration (r=-0.880),and MDA level (r=-0.862) (all Ps ＜ 0.01),respectively;the expression of PARK2 mRNA was positively correlated with SOD activity (r=0.879),DA level (r=0.859),and the expression of Parkin protein (r=0.809) (all Ps ＜ 0.01),respectively.[Conclusion] MnC12 exposure could induce mitochondrial damage,oxidative stress,and decreased DA secretion and expression of PARK2 in SH-SY5Y cells.关键词
氯化锰/人骨髓神经母细胞瘤细胞株/超氧化物歧化酶/丙二醛/多巴胺/PARK2Key words
manganese chloride/human bone marrow neuroblastoma cells/superoxide dismutase/malondialdehyde/dopamine/PARK2分类
医药卫生引用本文复制引用
范希敏,罗英,许洁,吴芹,刘杰,范奇元..氯化锰致人骨髓神经母细胞瘤细胞株线粒体损伤及对多巴胺分泌和PARK2表达的影响[J].环境与职业医学,2017,34(8):707-711,717,6.基金项目
国家自然科学基金(编号:81260420) （编号:81260420）
贵州省自然科学基金(编号:黔科合sy字[2012]3140) （编号:黔科合sy字[2012]3140）
遵义医学院基础药理省部共建教育部重点实验室开放课题(编号:2014-2) （编号:2014-2）
