南京医科大学学报（自然科学版）2017，Vol.37Issue(8)：966-970,5.DOI:10.7655/NYDXBNS20170809
皮质醇对激素依赖型乳腺癌细胞MCF-7增殖迁移影响及机制
Effect and mechanism of cortisol on proliferation and migration of hormone-related breast cancer MCF-7 cells
摘要
Abstract
Objective:To investigate the proliferation and migration effect of cortisol on hormone-related breast cancer MCF-7 cells.Methods:MCF-7 cells,originated from a hormone-related breast cancer woman,were treated with different concentration of cortisol.The effect of cortisol on cell proliferation was determined by MTS assay and the rate of cell survival was calculated.The effect of cortisol on cell migration was examined by wound-healing assay.Inmunoblot analysis detected the protein expression of phospho mTOR (p-mTOR),which is involved in cell proliferation,and MMP-9,MMP-2,which are related to cell migration.Results:Cortisol at concentration of 10 nmol/L significantly increased cell proliferation(P＜0.05)and cell migration(P＜0.01)compared with the vehicle group.Furthermore,cortisol at concentration of 10 nmol/L obviously increased the protein expression involved in cell proliferation (pmTOR,P＜0.05) and cell migration (MMP-9,P＜0.05;MMP-2,P＜0.01).Conclusion:Cortisol at concentration of 10 nmol/L effectively induced cell proliferation and cell migration by activating mTOR pathway and upregulating MMP-9 and MMP-2 protein expression.关键词
乳腺癌/皮质醇/激素依赖型Key words
breast cancer/cortisol/hormone-related分类
医药卫生引用本文复制引用
厉芝,甄林林,韩学东,施建华,任毅..皮质醇对激素依赖型乳腺癌细胞MCF-7增殖迁移影响及机制[J].南京医科大学学报（自然科学版）,2017,37(8):966-970,5.基金项目
江苏省“333高层次人才培养工程”(BRA2014129) （BRA2014129）
