南京医科大学学报（自然科学版）2017，Vol.37Issue(8)：985-989,5.DOI:10.7655/NYDXBNS20170813
IQGAP1在调节足细胞细胞骨架重组中的作用
The role of IQGAP1 in the regulation of cytoskeletal rearrangement in podocytes
张晓波 1唐凤英 1周敏 1朱颖 1陈敏 1周惠 1陈连华1
作者信息
- 1. 南京医科大学附属淮安第一医院肾内科,江苏淮安223300
- 折叠
摘要
Abstract
Objective:To investigate the regulatory role of IQGAP1 in cytoskeletal rearrangement of podocytes.Methods:Conditionally immortalized mouse podocytes were used to perform cell culture and stimulated by puromycin aminonucleoside (PAN).RT-PCR and Western blot analysis were used to measure the level of IQGAP1 mRNA and protein,respectively.The cytoskeleton of F-actin was evaluated by FITC-conjugated phalloidin staining under laser confocal scanning microscope and semi-quantitative system with cortical?F-actin score (CFS) was used to analyze the degree of actin cytoskeleton arrangement.We further explored the changes of cytoskeletal rearrangement after up-and down-regulation of IQGAP1.Results:①when compared with the control group,the expression of IQGAP1 mRNA and protein was significantly decreased after PAN stimulation in mouse podocytes (P＜0.05).PAN stimulation induced cytoskeletal rearrangement including cortical F-actin ring formation,stress fiber attenuation and CFS up-regulation;②the above rearrangement was exacerbated by IQGAP1 siRNA transfection (P＜0.05);③ PAN induced cortical F-actin ring formation and CFS up-regulation was abated after IQGAP1 plasmid transfection (P＜0.05).Conclusion:IQGAP1 can inhibit PAN induced cytoskeleton rearrangement in podocytes and plays an important role in maintaining normal podocyte cytoskeleton.关键词
IQGAP1/足细胞/细胞骨架重组/嘌呤霉素Key words
IQGAP1/podocytes/cytoskeleton rearrangement/puromycin aminonucleoside分类
医药卫生引用本文复制引用
张晓波,唐凤英,周敏,朱颖,陈敏,周惠,陈连华..IQGAP1在调节足细胞细胞骨架重组中的作用[J].南京医科大学学报（自然科学版）,2017,37(8):985-989,5.
