农业生物技术学报2017，Vol.25Issue(4)：607-613,7.DOI:10.3969/j.issn.1674-7968.2017.04.010
羊驼MC1R真核表达载体的构建及其在毛囊干细胞中的表达
Construction of Eukaryotic Expression Vector of MC1R Gene and Its Expression in Hair Follicle Stem Cells of Alpaca (Lama pacos)
摘要
Abstract
Melanocortin-1 receptor (MC1R) gene may play important roles in the regulation process of mammalian coat color,but the effects of exogenous MC1R gene on the micropht-halmiaassocitated transcription factor (MITF) and tyrosinase (TYR) genes in alpaca (Lama pacos) are less studied.To explore effect of MC1R gene on the expression of MITF and TYR,eukaryotic expression vector of MC1R gene pcDNA3.1 (+)-MC1R was constructed and transfected into alpaca hair follicle stem cells (HFSCs).The relative expression of MC1R,MITF and TYR mRNA were detected by qRT-PCR,and MC1R protein was located by immunocytochemistry,qRT-PCR results showed that the relative expression level of MC1R was significantly increased (P＜0.05) in the group transfected with pcDNA3.1(+)-MC1R (P＜0.05).There were 2.15 and 2.26 times than that in the group of pcDNA3.1(+) and the control group.MITF and TYR gene also had higher expression,but not significant (P＞0.05).Meanwhile immunocytochemistry results showed that MC1R protein had a positive expression in HFSCs.The results showed that the MC1R gene excessively expressed in alpaca HFSCs,and indirectly up-regulated the expression of TYR and MITF.This study provides scientific basis for further study on the expression mechanism of MC1R in the alpaca HFSCs.关键词
羊驼/载体构建/毛囊干细胞/黑色素皮质激素受体1基因(MC1R)Key words
Alpaca/Vector construction/Stem cells/Melanocortin-1 receptor gene (MC1R)分类
农业科技引用本文复制引用
南瑞鹏,陈娟娟,李振,卢杰丽,庞全海..羊驼MC1R真核表达载体的构建及其在毛囊干细胞中的表达[J].农业生物技术学报,2017,25(4):607-613,7.基金项目
国家自然科学基金项目(No.30972223和No.31272628) （No.30972223和No.31272628）
