山东中医药大学学报2017，Vol.41Issue(5)：462-466,5.
尖叶假龙胆不同提取部位对胰岛素抵抗H4ⅡE细胞葡萄糖消耗的影响
Effects of Different Fractions from Gmtianella Acuta on the Glucose Consumption of H4ⅡE Cells with Insulin Resistance
摘要
Abstract
Objective:To study the effects of different fractions from Gmtianella acuta influence on the glu-cose consumption of H4ⅡE cells with insulin resistance. Methods:The H4ⅡE cell activity was evaluated with method for the CCK-8. The H4ⅡE cells was cultured about 36 hours by insulin of high concentra-tion(10-6 mol/L) to establish new insulin resistance cell model. The effects of different fractions from Gm-tianella acuta on the glucose consumption of H4ⅡE cells with insulin resistance was tested by glucose oxi-dase. Results:The effects of different fractions from Gmtianella acuta on H4ⅡE cell activity were signifi-cantly inhibited,when their concentrations greater than 150μg/mL. The ethyl acetate fraction of Gmtianella acuta significantly affected the glucose consumption of H4ⅡE cells with insulin resistance. Macroporous resin-95% fraction and macroporous resin-70% fraction affected the glucose consumption of H4ⅡE cells with insulin resistance with different degrees,while macroporous resin-30% had no significant effects. Conclusion:The ethyl acetate fraction of Gmtianella acuta had best effects on insulin resistance,macroporous resin-95% fraction and macroporous resin-70% frac-tion could improve the insulin resistance with dif-ferent degrees.关键词
尖叶假龙胆/H4ⅡE细胞/胰岛素抵抗/葡萄糖消耗Key words
Gmtianella acuta/H4ⅡE cells/insulin resistance/glucose consumption分类
医药卫生引用本文复制引用
高佳琪,刘铜华,魏颖,王东超,石浩霞,李博,秦灵灵,孙文,兰卫,徐暾海..尖叶假龙胆不同提取部位对胰岛素抵抗H4ⅡE细胞葡萄糖消耗的影响[J].山东中医药大学学报,2017,41(5):462-466,5.基金项目
国家重大新药创制(编号:1000071320025) （编号:1000071320025）
