山东医药2017，Vol.57Issue(34)：1-4,4.DOI:10.3969/j.issn.1002-266X.2017.34.001
长链非编码RNA CCAT2对肝癌细胞增殖和侵袭迁移能力的影响及其机制探讨
Effects of lncRNA CCAT2 on proliferation, invasion, and migration of hepatocellular carcinoma cells
摘要
Abstract
Objective To observe the role and mechanism of lncRNA CCAT2 in proliferation, invasion and migration of hepatocellular carcinoma (HCC) cells.Methods HepG2 cells in logarithmic phase were randomly divided into groups A, B, and C, which were transiently transfected with control plasmids, small interfering RNA (si-CCAT2), and si-CCAT2+EZH2 overexpression plasmid by using Lipofectamine 3000.The real-time PCR was used to detect the expression of lncRNA CCAT2 and EZH2 mRNA.Western blotting was used to detect the protein level of EZH2.MTT method was used to observe the proliferation of cells at 24, 48, 72 and 96 h (OD490).Scratch test and Transwell assay were used to detect cell migration and invasion.Results Compared with group A, the mRNA and protein expression of lncRNA CCAT2 and EZH2 decreased in the group B (both P<0.05).Compared with group B, the mRNA and protien levels of EZH2 increased in the group C (both P<0.05).The OD490 value representing cell proliferation was lower in the group B than that of the group A, and the OD490 value in the group C was higher than that of the group B (all P<0.05).The percentage of wound healing and the number of penetrating cells in the group B were lower than those of the group A (both P<0.05);compared with group B, the percentage of wound healing and the number of penetrating cells were higher in the group C than in the group B (both P<0.05).Conclusions LncRNA CCAT2 can promote the proliferation, invasion, and migration of HCC cells by up-regulating the expression of EZH2, and thus promote the occurrence and development of HCC.关键词
肝细胞癌/长链非编码RNA CCAT2/EZH2基因/细胞增殖/细胞侵袭/细胞迁移Key words
hepatocellular carcinoma/long non-coding RNA CCAT2/EZH2 gene/cell proliferation/cell invasion/cell migration分类
医药卫生引用本文复制引用
纪易斐,刘金霞,江枫,刘肇修,管程齐,肖明兵,倪润洲..长链非编码RNA CCAT2对肝癌细胞增殖和侵袭迁移能力的影响及其机制探讨[J].山东医药,2017,57(34):1-4,4.基金项目
国家自然科学基金资助项目(81502055). （81502055）
