摘要
Abstract
Objective To observe the effect of silencing cellular inhibitor of apoptosis protein 1 (c-IAP1) on the sensitivity of pancreatic cancer HS766T cells to radiotherapy.Methods HS766T cells were randomly divided into the control group, empty vector group, and observation group.The observation group and the empty vector group were transfected with silencing cIAP-1 gene plasmid cIAP-1-RNAi and negative gene plasmid NC-RNAi, and the control group was not transfected.The cIAP-1 mRNA and protein levels of cells were detected by real-time PCR and Western blotting.The HS766T cells with stably silenced cIAP-1 in the observation group and HS766T cells in the control group were collected to determine the survival rate of cells after 2, 4, 6 and 8 Gy X ray irradiation for 24 h by CCK-8, as well as the apoptotic rate of cells after 6 Gy X ray irradiation for 12, 24 and 48 h by flow cytometry.Results The relative expression of cIAP-1 mRNA and protein in the observation group was lower than that in the empty vector group and the control group (P<0.05), and there was no significant difference in the relative expression of cIAP-1 mRNA and protein between the empty vector group and the control group.With the increase of radiation dose, the survival rate of cells decreased;under the same irradiation dose, the cell survival rate in observation group was lower than that in the empty vector group and the control group (P<0.05), and the cell survival rate showed no significant difference between the empty vector and control group.Over the time of irradiation, the apoptotic rate showed an increasing trend;at the same time point, the apoptotic rate of cells in the observation group was higher than that of the empty vector group and the control group (P<0.05), and there was no significant difference between the empty vector and control group.Conclusion Silencing cIAP-1 gene expression can inhibit the survival of pancreatic cancer cells, promote apoptosis and enhance the sensitivity of pancreatic cancer cells to radiotherapy.关键词
胰腺癌/细胞凋亡抑制蛋白1/HS766T细胞/放射疗法/敏感性Key words
pancreatic carcinoma/cellular inhibitor of apoptosis protein 1/HS766T cells/radiotherapy/sensitivity分类
医药卫生
