山东医药2017，Vol.57Issue(34)：16-19,4.DOI:10.3969/j.issn.1002-266X.2017.34.005
广藿香醇对幽门螺杆菌酸抵抗能力的影响及其机制探讨
Effect of patchouli alcohol on acid resistance of Helicobacter pylori
摘要
Abstract
Objective To investigate the effect of patchouli alcohol on acid resistance of Helicobater pylori (Hp) and its mechanism.Methods After Hp were cultured in vitro and identified, Hp were divided into 5 groups.The high-, medium-, and low-dose drug groups were added with 20, 10, and 5 mg/L patchouli alcohol;the positive control group was added with urea enzyme inhibitor AHA;the blank control group was added with the same amount of DMSO.Agar dilution method was used to detect the bacterial survival rates in the acidic (pH=1.0) and neutral (pH=7.0) conditions after 0.5 hour.We obtained the Hp urease protein A (UreA) , urease protein B (UreB), and urease protein G (UreG) by E.coli expression system, and mixed up UreA and UreB protein.Then, we took the UreA/UreB protein mixed solution to simulate the Ure maturation process and mixed the UreG protein monomer solution with the nickel ion (Ni2+) solution.The Ure activity was detected by Berthelot method to determine the Ure maturity.The concentration of Ni2+ was measured by atomic absorption spectrometry to determine the ability of UreG carrying Ni2+.Results In the neutral condition, there was no significant difference in the Hp colony forming units among the high-, medium-, and low-dose drug groups, positive control group, and blank control group.Compared with the blank control group, the Hp colony forming units of the high-, medium-, and low-dose drug groups and positive control group decreased in neutral conditions (all P<0.05), and the order was as follows: the high-dose drug group, positive control group < medium-dose drug group < low-dose drug group (all P<0.01).The urease activities of the high-, medium-, low-dose drug groups and the control group were 54.89％±11.47％, 63.99％±9.46％, 83.88％±11.81％, and 101.53％±2.55％;the concentrations of Ni2+ were (0.77±0.24), (0.80±0.22), (1.12±0.35), (1.56±0.42) μmol/L, and the order was as follows: the high-dose drug group, medium-dose drug group < low-dose drug group < control group (all P<0.01).Conclusions Patchouli alcohol can decrease the acid resistance of Hp, which may be associated with blocking the transmission pathway of Ni2+ and Ure''s maturation process and thus inhibiting Ure activity.关键词
幽门螺杆菌/酸抵抗/广藿香醇/尿素酶成熟/镍离子传递Key words
Helicobacter pylori/acid resistance/patchouli alcohol/urease mature/nickel ion transfer分类
医药卫生引用本文复制引用
连大卫,许艺飞,扶丽君,任文康,魏文辉,庄惠玲,黄萍,操红缨..广藿香醇对幽门螺杆菌酸抵抗能力的影响及其机制探讨[J].山东医药,2017,57(34):16-19,4.基金项目
国家自然科学基金资助项目(81374043) （81374043）
广东省科技计划资助项目(2016A020217019) （2016A020217019）
广东省广州市科技计划资助项目(201607010336). （201607010336）
