山东医药2017，Vol.57Issue(34)：20-23,4.DOI:10.3969/j.issn.1002-266X.2017.34.006
痛泻要方对肝郁脾虚型溃疡性结肠炎大鼠结肠组织中gp130、SOCS3表达的影响
Effects of Tongxie Yaofang on expression of gp130 and SOCS3 in rats with liver depression and spleen deficiency type ulcerative colitis
摘要
Abstract
Objective To observe the effects of Tongxie Yaofang (Tongxie prescription) on the expression of glycoprotein 130 (gp130) and suppressor of cytokine signaling 3 (SOCS3) gene and protein in colonic tissues of rats with liver depression and spleen deficiency type ulcerative colitis (UC).Methods Sixty rats were randomly divided into groups A, B, C, and D.Rats in the groups B, C, and D were treated with trinitrobenzene sulfonic acid/ethanol solution + restraint stress and improper diet to induce the liver depression and spleen deficiency type UC models.Rats in the group A were treated with the same method by using the same amount of normal saline enema.Rats in the groups C and D were treated with Tongxie Yaofang (crude drug 5.5 g/kg), mesalazine (0.5 g/kg) 10 mL/kg by gavage.Rats in the groups A and B were fed with the same volume of normal saline.After the success of modeling in each group, the rats were treated by gavage, once a day for 21 days.We removed the colon segment from 7 to 8 cm above the anus, and determined the colon mucosal injury score by naked eyes.Real-time fluorescent quantitative PCR, immunohistochemistry, and immunoblotting were used to detect gp130, SOCS3 gene and protein in colon tissues.Results The score of colonic mucosal injury in the group B was 2.67±0.62, which was higher than that of the group A (0.6±0.63) (P<0.01);the score of colonic mucosal injury in the group C and group D was 1.73±0.70 and 1.27±0.46, which was both higher than that of the group B (P<0.01).There was no significant difference between groups C and D.Compared with group A, the expression of gp130 gene and protein in the group B was higher and the expression of SOCS3 gene and protein was lower (all P<0.05).Compared with group B, the expression of gp130 gene and protein in the groups C and D was lower and the expression of SOCS3 gene and protein was higher than that of group B (all P<0.05).There was no significant difference in gp130 and SOCS3 gene and protein between the groups C and D.Conclusion Tongxie Yaofang can inhibit the expression of gp130 and up-regulate the expression of SOCS3, so as to improve the degree of colonic mucosal injury of rats with liver depression and spleen deficiency type UC.关键词
溃疡性结肠炎/痛泻要方/美沙拉秦/糖蛋白130/细胞因子信号传导抑制蛋白3/大鼠Key words
ulcerative colitis/Tongxie Yaofang/mesalazine/glycoprotein 130/suppressor of cytokine signaling 3/rats分类
医药卫生引用本文复制引用
杨意,朱向东,翟艳会,李婷..痛泻要方对肝郁脾虚型溃疡性结肠炎大鼠结肠组织中gp130、SOCS3表达的影响[J].山东医药,2017,57(34):20-23,4.基金项目
国家自然科学基金资助项目(81460696). （81460696）
