山东医药2017，Vol.57Issue(34)：24-26,3.DOI:10.3969/j.issn.1002-266X.2017.34.007
CD80、CD86抗体对耗竭性T细胞效应功能的影响
Influence of CD80 and CD86 antibody on effector function of exhausted T cells
摘要
Abstract
Objective To investigate the effects of CD80 and CD86 antibody on the effector function of exhausted T cells.Methods Forty-two female HLA-A11DR1 transgenic mice were randomly divided into 7 groups (n=6).The mice in groups A-F were subcutaneously inoculated with phosphatidylinositol 3 (GPC3) at the age of 8 weeks (week 8), at week 11 and week 14, respectively.Mice in the group G were inoculated with the same volume of heat-free PBS as controls.Mice in the groups A-E were sacrificed at week 24, and groups F and G at week 17.Splenocytes suspension was made.Then, 20 or 40 μg/mL CD80 antibody and 10 μg/mL GPC3 peptide were pipetted in the splenocytes of groups A and B;20 or 40 μg/mL CD86 antibody and 10 μg/mL GPC3 were pipetted in the groups C and D, respectively;10 μg/mL GPC3 peptide was pipetted in the groups E, F, and G.After incubation for 18 h, interferon-γ (IFN-γ) was measured by enzyme-linked spot analyzer for analysis of T cell function.Results IFN-γ positive spots in the groups A-G were 80.61±48.91, 207.67±60.41, 1.67±0.97, 1.33±0.49, 2.33±1.53, 38.17±5.18 and 2.33±1.53, respectively.Among them, group F was significantly higher than groups E and G (both P<0.01).There was no significant difference between groups E and G (both P<0.01).Group A was more than group B, and group B was significantly more than group C, D and E (all P<0.01).There was no significant difference among groups C, D and E.In addition, the number of IFN-γ positive spots was positively correlated with CD80 antibody concentration (r=0.7605, P<0.01).Conclusions Anti-CD80 could restore the exhausted T cells to secret IFN-γ with a dose-dependent manner, while anti-CD86 could not be detected for the same effect.关键词
CD80抗体/CD86抗体/磷脂酰肌醇蛋白聚糖3/多肽疫苗/T细胞耗竭/干扰素γKey words
CD80 antibody/CD86 antibody/phosphatidylinositol 3/peptide vaccine/T cell exhaustion/interferon-γ分类
医药卫生引用本文复制引用
孙斌,杨晓珍,郭向华,周育森,孙世惠,王延军..CD80、CD86抗体对耗竭性T细胞效应功能的影响[J].山东医药,2017,57(34):24-26,3.基金项目
北京市科学技术委员会首都市民健康项目(Z141100002114002) （Z141100002114002）
北京市卫生系统高层次卫生技术人才项目(2014-3-092). （2014-3-092）
