山东医药2017，Vol.57Issue(34)：27-29,3.DOI:10.3969/j.issn.1002-266X.2017.34.008
刺参酸性黏多糖对荷瘤小鼠肝癌组织中细胞凋亡相关蛋白表达的影响
Effects of Stichopus japonicus acid mucopolysaccharide on apoptosis-related protein expression in liver cancer tissues of tumor-bearing mice
摘要
Abstract
Objective To observe the effects of Stichopus japonicus acidic mucopolysaccharide (SJAMP) on apoptosis-related protein expression in liver cancer tissues of tumor-bearing mice.Methods Fifty hepatocarcinoma-bearing mice were established by subcutaneous injection of tumor cells in the right axillary, and then they were randomly divided into the negative control group (injected with normal saline), positive control group (injected with 5-FU 20 mg/kg), low-dose SJAMP group (6.25 mg/kg SJAMP 0.2 mL), medium-dose SJAMP group (12.5 mg/kg SJAMP 0.2 mL), and high-dose SJAMP group (25 mg/kg SJAMP 0.2 mL).The mice were all intervened for 12 days.The mice were sacrificed, and we weighed the tumor and calculated the tumor inhibition rate.HE staining was used to observe the pathological changes of tumor tissues.The expression of Caspase-3, Caspase-9, cytochrome C, Smac protein, survivin and NF-κB was detected by immunohistochemistry.Results The tumor inhibition rate of the high-dose SJAMP group was higher than that of the low-dose and medium-dose SJAMP groups (P<0.05).The decreased cell density and nuclear division as well as increased necrotic area were found in the positive control group, medium-dose and high-dose SJAMP groups.Compared with the negative control group, the expression of Caspase-3, Caspase-9, cytochrome C,and Smac protein increased in the medium-dose, high-dose SJAMP groups and positive control group (P<0.05), while the expression of survivin and NF-κB significantly decreased (all P<0.05).Conclusion The medium-dose and high-dose SJAMP can effectively up-regulate the expression of pro-apoptotic gene, down-regulate the expression of apoptosis inhibiting gene, thus inhibit the growth of tumor-bearing mice.关键词
肝癌/刺参酸性黏多糖/细胞凋亡/含半胱氨酸的天冬氨酸蛋白水解酶/细胞色素C/Smac蛋白/存活素/核转录因子κB/小鼠Key words
liver carcinoma/Stichopus japonicus acidic mucopolysaccharide/Caspase/cytochrome C/Smac protein/survivin/nuclear transcription factor κB/mice分类
医药卫生引用本文复制引用
李晓娟,宋扬,陈丹丹,代海华..刺参酸性黏多糖对荷瘤小鼠肝癌组织中细胞凋亡相关蛋白表达的影响[J].山东医药,2017,57(34):27-29,3.基金项目
国家自然科学基金资助项目(81072295). （81072295）
