实验动物与比较医学2017，Vol.37Issue(4)：257-265,9.DOI:10.3969/j.issn.1674-5817.2017.04.001
人胃癌细胞系的建立及其评价
Establishment and Characterization of Patient Derived Gastric Cancer Cell Lines
摘要
Abstract
Objective To establish and characterize primary gastric cancer cell lines derived from gastric cancer patient-derived xenograft (PDX) models.Methods Tumors from 12 stable gastric cancer PDX models were selected to establish primary tumor cell lines when the tumor volume reached 500～900 mm3.The established cell lines were characterized by cell morphology,chromosome,STR and immunohistochemical analyses,in vivo tumorigenicity,as well as drug sensitivity in vitro and in vivo.Results Six primary gastric tumor cell lines were successfully established.GAXC031 and GAXC066 were selected and characterized thoroughly.STR analysis confirmed that these 2 cell lines were consistent with their parental PDX models.These 2 cell lines presented the morphological,histological and genomic characteristics of malignant gastric cancer cells.They generated xenograft readily in nude mice and these tumors maintained histology characteristics of the parental PDX tumors.Moreover,compared to the parental PDX models,these 2 cell lines initiated xenografts with higher take rate,shorter incubation period,and better uniformity in tumor growth.In vitro and in vivo drug sensitivity test showed that GAXC031 was more sensitive to 5-fluorouracil than that of GAXC066,while both cell lines were sensitive to cisplatin.Conclusions Six patient derived gastric cell lines were successfully established from PDX models in this study.Deep characterization showed that these cell lines maintained many characteristics of the original PDX models with improved take rate,latent period and uniformity,therefore represent useful tools in basic research and drug screening.关键词
人源化异种移植瘤(PDX)/胃癌/细胞系/生物学特性/药效学实验Key words
Patient-derived xenograft (PDX)/Gastric cancer/Cell lines/Biological characteristics/Drug efficacy study分类
生物科学引用本文复制引用
葛晓梅,刘喜朋,周禾,强福林,张一心,谢付波,刘继斌,杨磊,渠颖颖,顾莹,李学廷,杨伟敏..人胃癌细胞系的建立及其评价[J].实验动物与比较医学,2017,37(4):257-265,9.基金项目
科技部国家科技支撑计划项目(2015BAH24F00) （2015BAH24F00）
