实验动物与比较医学2017，Vol.37Issue(4)：315-319,5.DOI:10.3969/j.issn.1674-5817.2017.04.012
香烟烟雾对慢性阻塞性肺疾病大鼠肺功能及血清细胞因子水平影响
Effects of Cigarette Smoke on Lung Function and Serum Cytokine Levels in Chronic Obstructive Pulmonary Disease Rat
摘要
Abstract
Objective To investigate the influence of cigarette smoke on lung function and serum cytokine levels in chronic obstructive pulmonary disease (COPD) rats which were established by the complex stimulation of lipopolysaccharide (LPS) and smoked cigarettes.Methods Seventy-two male SD rats were randomly divided into six groups,4 weeks,6 weeks and 8 weeks model group,3 weeks and 6 weeks natural healing group,and control group.A COPD rat model was established by the complex stimulation of two times LPS injection and smoked cigarettes.The lung function of the rats was measured during passive smoking and quit smoking.At the same time serum interleukin-8(IL-8),IL-10 and tumour necrosis factor-alpha (TNF-α) levels were monitored by Enzyme-linked immunosorbent assay.Correlation analysis concerning cytokine levels and pulmonary function index were conducted.Results Compared with control group,forced expiratory volume in 0.3 second (FEV0.3),forced expiratory volume in 0.3 second as a percentage of forced vital capacity (FEV0.3/FVC) and IL-10 were decreased (P＜0.05),IL-8 and TNF-α levels were increased in the model groups (P＜0.05).And as the building time prolonged,the changes of those cytokines and lung function indexes were more obvious than before.IL-8 and TNF-α levels were inversely associated with FEV0.3/FVC(P＜0.001,r=-0.579;P=0.034,r=-0.354).Compared with control group,IL-8 and TNF-α levels were decreased in the 6 weeks healing group,at the same time IL-10 levels was increased(P＜0.05),the FEV0.3 and FEV0.3/FVC were decreased in the healing groups (P＜0.05).Conclusions Cigarette smoke is the primary cause of changes on cytokine levels in this animal model.关键词
慢性阻塞性肺疾病(COPD)/细胞因子/动物模型/香烟烟雾Key words
Chronic obstructive pulmonary disease (COPD)/Cytokines/Animal model/Cigarette smoke分类
医药卫生引用本文复制引用
熊英,张金娟,李玲,陈燕,吴艳俊,黄惠妍..香烟烟雾对慢性阻塞性肺疾病大鼠肺功能及血清细胞因子水平影响[J].实验动物与比较医学,2017,37(4):315-319,5.基金项目
贵州省科学技术基金(黔科合LG字[2012]014号) （黔科合LG字[2012]014号）
