同济大学学报（医学版）2017，Vol.38Issue(4)：1-5,12,6.DOI:10.16118/j.1008-0392.2017.04.001
MTA3通过β-hCG调控上皮性卵巢癌侵袭转移的机制研究
Expression of MTA3 in epithelial ovarian cancer and its relation to tumor invasion and metastasis
摘要
Abstract
Objective To investigate the expression of metastasis associated gene 3 (MTA3) in epithelial ovarian cancer (EOC) and its relation to tumor invasion and metastasis.Methods The frozen tissue (8 cases) and paraffin embedded tissue (21 cases) specimens of advanced EOC (FIGO Ⅲ-Ⅳ) were collected.The expression of MTA3 in ovarian cancers tissues and metastasis tissues were detected by qRT-PCR and immunohistochemical staining (IHC).Human epithelial ovarian carcinoma SKOV3 and ES-2 cells were transfected with siRNA-MTA3,siRNA-β-hCG,both siRNA-MTA3 and siRNA-β-hCG,respectively.The migration of transfected SKOV3 and ES-2 cells was examined by Transwell assay.EOC SKOV3 and ES-2 cells were transfected with 50,100,200 nmol/L siRNA-MTA3 and qRT-PCR was used to detect the correlation of MTA3 and β-hCG.The mRNA and protein expression of β-hCG,E-cadherin and N-cadherin was detected by qRT-PCR and Western blotting after down-regulated MTA3.Results The expression of MTA3 in metastatic tissue of ovarian cancer was significantly lower than that in primary ovarian tumor (P<0.05).Transwell assay showed that the migration ability of EOC cells was significantly enhanced after down-regulation of MTA3;while it was decreased after down-regulation of β-hCG;when MTA3 and β-hCG were both down-regulated the cell migration ability was markedly decreased (P<0.001).qRT-PCR showed a significant negative correlation between the expression of MTA3 and β-hCG in EOC cell lines (P<0.05).qRT-PCR and Western blotting showed that down-regulation of MTA3 reduced the epithelial markers E-cadherin,while increased the expression of β-hCG and mesenchymal marker N-cadherin.Conclusion MTA3 may up-regulate β-hCG expression to enhance the invasion and metastasis in epithelial ovarian carcinoma through epithelial-mesenchymal transition.关键词
上皮性卵巢癌/MTA3/β-hCG/EMT/肿瘤转移Key words
epithelial ovarian cancer/MTA3/β-hCG/EMT/tumor metastasis分类
医药卫生引用本文复制引用
彭书敏,王凯,詹光熙,美丽古丽·莫合买提,郭晓青..MTA3通过β-hCG调控上皮性卵巢癌侵袭转移的机制研究[J].同济大学学报（医学版）,2017,38(4):1-5,12,6.基金项目
国家自然科学基金面上项目(81372305) （81372305）
