同济大学学报（医学版）2017，Vol.38Issue(4)：6-12,7.DOI:10.16118/j.1008-0392.2017.04.002
单核细胞来源的微颗粒通过PPAR-α-SR-BI途径促进RAW264.7泡沫细胞形成的研究
Monocyte-derived microparticles promote RAW264.7 foam cell formation via the PPAR-α-SR-BI pathway
摘要
Abstract
Objective To investigate the role of the monocyte-derived microparticles (mono-MPs) in the formation of foam cells.Methods Mouse macrophage RAW264.7 cells were stimulated with oxidized low density lipoprotein or low density lipoprotein to form foam cells.Positive foam cells were stained with oil red staining.Real-time PCR and Western blotting were used to determine RAW264.7 Cell receptor or enzyme CD36,SR-A,ACAT1,nCEH,ABCA1,ABCG1,SR-BI.Results Mono-MPs affected the activity of RAW264.7 cells.Mono-MPs promoted the formation of RAW264.7 foam cells;low concentration (20μl/ml) microparticles slightly promoted the phagocytosis of macrophages to low-density lipoproteins,but did not affect the intake of oxidized low-density lipoproteins.Peroxisome proliferator-activated receptor-alpha (PPAR-α) agonists enhanced foam cells formation,while the effect of PPAR-α antagonist was reversed.Mono-MPs reduced the expression of SR-BI mRNA and protein levels.PPAR-α agonists reduced the expression of SR-BI protein in RAW264.7 macrophages,while PPAR-α antagonist had no effect.Conclusion The mono-MPs,causing activation of the PPAR-α,induce the RAW264.7 foam cell formation via lipid uptake and the PPAR-α-SR-BI pathway.关键词
单核细胞微颗粒/巨噬细胞/泡沫细胞/过氧化物酶体增殖物激活受体-α/动脉粥样硬化Key words
monocyte-derived microparticles/macrophage/foam cell/PPAR-α/atherosclerosis分类
医药卫生引用本文复制引用
刘少飞,匡雅姝,彭盛,闫建设,李莹..单核细胞来源的微颗粒通过PPAR-α-SR-BI途径促进RAW264.7泡沫细胞形成的研究[J].同济大学学报（医学版）,2017,38(4):6-12,7.基金项目
上海市浦东新区科委课题(PKJ2013-Y19) （PKJ2013-Y19）
