同济大学学报（医学版）2017，Vol.38Issue(4)：21-25,5.DOI:10.16118/j.1008-0392.2017.04.005
Runx2在人退变腰椎间盘软骨终板中的表达及意义
Expression of Runx2 in degenerated lumbar endplate cartilage and its clinical significance
摘要
Abstract
Objective To investigate the expression of Runx2 in degenerated lumbar endplate cartilage and its clinical significance.Methods Twenty two specimens of degenerated human lumbar cartilage endplates (study group) and 20 specimens of non-degenerated lumbar cartilage endplate (control group) were collected.H-E staining was used to examine the morphological changes of lumbar cartilage endplate.The expression of Runx2 protein and mRNA was detected with immunohistochemistry,RT-PCR and Western blotting,respectively;the expression of aggrecan and collagen I was also detected with Western blotting in two groups.Results The structure of cartilage endplate was complete and compactly arranged in control group,while in the degenerated group the structure of endplate was seriously destructed.Immunohistochemistry analysis showed that the Runx2-positive cells were scarcely distributed in normal endplates,while significantly widely distributed in the degenerated endplates.In the mild,moderate and severe degenerative groups,RT-PCR analysis showed that the mRNA expression of Runx2 was positively correlated with the degenerative degree of endplate cartilage,which was (4.74±0.78),(7.74±0.97) and (16.97±1.89) fold higher than that of control group,respectively.Western-blotting showed that the expressions of Runx2,aggrecan and collagen I protein in mild,moderate and severe degenerative group were (2.79±0.52),(0.48±0.12),(0.67±0.17) fold (P<0.05);(0.36±0.12),(0.23±0.14),(4.40±1.78) fold (P<0.01);and (8.75±1.88),(0.13±0.09),(0.08±0.03) fold (P<0.01) higher than those in control group.Conclusion Runx2 can be an ideal indicator to predict the degeneration degree of lumbar endplate cartilage and may serve as a potential treatment target for lumbar disc degenerative diseases.关键词
椎间盘退变/腰椎/软骨终板退变/Runt相关转录因子2Key words
intervertebral disc degeneration/lumbar vertebra/endplate cartilage degeneration/Runx2分类
医药卫生引用本文复制引用
胡舟扬,李新华,潘杰,杨明杰,赵卫东,李立钧..Runx2在人退变腰椎间盘软骨终板中的表达及意义[J].同济大学学报（医学版）,2017,38(4):21-25,5.基金项目
上海市卫生与计划委员会科研项目(201640063) （201640063）
江西省自然科学基金项目(20171BAB205034) （20171BAB205034）
