同济大学学报（医学版）2017，Vol.38Issue(4)：49-54,59,7.DOI:10.16118/j.1008-0392.2017.04.011
母婴传播中慢性乙型重型肝炎病毒相关变异的研究
Evolutionary tendency of chronic severe hepatitis B-related mutations in mother-to-child transmission of hepatitis B virus
摘要
Abstract
Objective To study the evolutionary tendency of chronic severe hepatitis B (CSHB)-related mutations in the preS region of hepatitis B virus (HBV) during mother-to-child transmission.Methods Peripheral blood from HBsAg-positive mothers,umbilical cord blood from newborns,and peripheral blood from 7-month old infants who received intact vaccination were collected.Cloning sequencing was conducted to detect the viral mutations.Genotyping and phylogenetics analysis were conducted to determine the mother-to-child transmission of HBV.The Pearson Chi-square test and continuity correction were applied to evaluate the categorical variables.Results HBV strains with CSHB-related mutations C2875T,C2980T,G2988A,C3067T,C3097A,T25G,A76C,A79G,T100C,C102T,C106A,T109G,C135T,T147C and A148G could be transmitted to newborns through intrauterine pathway.HBV mutations includingA3097C/G and G132C had survival advantages in intrauterine transmission and chronicity (P<0.05;P<0.05).Genotype B2 HBV strains with mutations C3057G,T3060C,C3097A,T3169A,A20G,A76C,and C129T could be transmitted to 7-month old infants through mother to child transmission,while the similar phenomenon was not observed in genotype C2 HBV strains.Conclusion More CSHB-related mutations could be detected in genotype B2 HBV during mother-to-child transmission.After the immunization,HBV without the CSHB-related mutations has advantage of infecting infants and reflects the conservative nature on evolution perspectives of HBV.关键词
乙型肝炎病毒/慢性乙型重型肝炎/变异/宫内感染/母婴传播Key words
hepatitis B virus/chronic severe hepatitis B/mutations/trans-placental HBV transmission/mother-to-child transmission分类
医药卫生引用本文复制引用
刘敏,李自雄,路卫,李怀芳,曹广文..母婴传播中慢性乙型重型肝炎病毒相关变异的研究[J].同济大学学报（医学版）,2017,38(4):49-54,59,7.基金项目
国家自然科学基金重点国际合作项目(81520108021) （81520108021）
