摘要
Abstract
Objective To evaluate the application of T-Spot test for diagnosis of tuberculosis.Methods The clinical and laboratory data of 479 patients,including 279 with pulmonary tuberculosis,89 with extrapulmonary tuberculosis and 111 non-tuberculosis patients (control group),admitted in Pulmonary Hospital,Tongji University from January 2016 to September 2016 were retrospectively reviewed.T-spot test,fluorescent staining,bacterial culture of sputum,TB-Sat and the TB-Ab test were performed in all patients.The sensitivity,specificity,positive predictive value and negative predictive value of tests were analyzed.Results The sensitivity,specificity,negative predictive value and positive predictive value of T-Spot test for diagnosis of tuberculosis were 96.47%(355/368),78.38%(87/111),93.67% (355/379)and 87%(87/100),respectively.The sensitivity of T-Spot in diagnosis of pulmonary tuberculosis and extrapulmonary tuberculosis were 96.77%(270/279)and 95.5%(85/89)respectively(P>0.05) and the specificity was 78.38%(87/111).The sensitivity of T-Spot in diagnosis of tuberculosis was higher than that of fluorescent staining,bacterial culture of sputum,TB-Sat and TB-Ab test(P<0.05).Compared to single tests,the sensitivity of combined detection of T-Spot and TB-Ab test was increased to 98.10% (361/368,P<0.05).The sensitivity of T-Spot in the patients with bacterial negative tuberculosis was 95.48% (148/155),and the specificity was 92.47% (86/93);and there was no significant difference between the patients with pulmonary tuberculosis and extrapulmonary tuberculosis.Conclusion T-Spot test is effective in detection of tuberculosis,particularly for bacteria negative patients;and combined T-Spot with TB-Ab test can improve the detection rate.关键词
T-Spot试验/结核/肺外结核/诊断Key words
T-Spot test/tuberculosis/outer-pulmonary tuberculosis/diagnosis分类
医药卫生
