森林工程2017，Vol.33Issue(5)：29-34,6.
粗毛纤孔菌三萜初级纯化及体外降血脂研究
Primary Purification and in vitro Hypolipidemic Activity ofTriterpenoid from Inonotus hispidus
摘要
Abstract
In order to study the primary purification process of triterpenoid from Inonotus hispidus and their effects in vitro hypolipidemic activity,triterpenoid from Inonotus hispidus was selected as raw material and macroporous resin was used to screen the best purification conditions.Then,the effect of purified triterpeniod combined with bile salt in vitro hypolipidemic activity was studied.The results showed that D-101 resin was the best,and its dynamic purification conditions were as follows:the column flow rate was 0.25 mL/min,loading mass concentration was 2.0 mg/mL,pH was 6;elution flow rate was 1 mL/min,ethanol volume fraction was 80％,elution volume was 4BV.Under these conditions,the purity of triterpenoids was 35.4％,which was 5.36 times higher than that of the crude extract.The experiment result of bile salts-combined in vitro indicated that,the binding amount before purification was 1.31±0.09μmol/100 mg,the after purification was 1.58±0.06μmol/100 mg,which was 46.33％ and 58.4％ of simvastatin respectively.The preliminary conclusion was that the hypolipidemic activities of terpenoid from Inonotus hispidus was positively related to their purity,and the purification process can improve the hypolipidemic activities.The results would provide a scientific basis for the development of new highly purified hypolipidemic drugs.关键词
粗毛纤孔菌/三萜类化合物/纯化/大孔树脂/降血脂Key words
Inonotus hispidus/triterpeniod/purification/macroporous resin/hypolipidemic activity分类
农业科技引用本文复制引用
杜令娟,康宁,李德海,周聪,顾嘉琳,王占斌..粗毛纤孔菌三萜初级纯化及体外降血脂研究[J].森林工程,2017,33(5):29-34,6.基金项目
中央高校基本科研业务费专项资金资助(2572014CA19) （2572014CA19）
黑龙江省自然科学基金面上项目(C2015062) （C2015062）
