生态学报2017，Vol.37Issue(12)：4217-4226,10.DOI:10.5846/stxb201605200974
温度和营养盐限制对网状原角藻生长与产毒的影响
Effects of temperature and nutrient limitation on growth and yessotoxin production of Protoceratium reticulatum
摘要
Abstract
Protoceratium reticulatum,a marine dinoflagellate that produces yessotoxins (YTXs),is common in coastal waters worldwide and can form toxic algal blooms.There are marked differences in its growth and toxin-producing characteristics in different areas.This study investigated the effects of temperature and nutrient (N,P) limitation on the growth and toxin production of P.reticulatum recently isolated from the North Yellow Sea.The results showed that temperature and nutrient limitation affected growth and toxin production of the alga to different degrees,but nutrient limitation had a more significant influence.Lower temperatures were more suitable for the growth of P.reticulatum.Growth of algal cells in L1-Si medium without nutrient limitation was best at 15℃.P limitation greatly reduced the specific growth rate and cell density (P ＜ 0.01),and shortened the duration of the exponential and stationary phases.N-limited cultures,in contrast,resulted in a growth rate and maximum cell density similar to those in L1-Si medium,but with a shorter stationary phase.At all temperatures,both N and P limitation were favorable for the accumulation of YTX in algal cells,which was particularly marked in the latter.The maximum YTX content of 92.6pg/cell was observed in 1/10P medium at 15℃ at the end of the stationary phase.This was approximately 3.8 times and 7.1 times that of the YTX in the N limited and L1-Si medium at the same culture temperature,respectively.Changes in temperature had different influences on the content of intracellular YTX in N-and P-limitation cultures.In the range of 5-15℃,temperature change had a negative effect on YTX increase under N limitation,whereas in P-limited medium,the effect was positive.In all culture conditions,the YTX concentration in the filtrate began to increase during the stationary phase.Compared with L1-Si,both N-and P-limited media were not conducive to the release of toxins.Higher temperature helpful to YTX release in the L1-Si and N-limited media,but it did not significantly affect that in the P-limited medium.关键词
网状原角藻/营养盐限制/温度/虾夷扇贝毒素/北黄海Key words
Protoceratium reticulatum/nutrient limitation/temperature/yessotoxin (YTX)/North Yellow Sea引用本文复制引用
高春蕾,孙萍,贾智慧,姜美洁,赵翠琼,张学雷,吴振兴,梁成珠..温度和营养盐限制对网状原角藻生长与产毒的影响[J].生态学报,2017,37(12):4217-4226,10.基金项目
国家自然科学基金(41206161) （41206161）
海洋公益性行业科研专项经费项目(201305010) （201305010）
国家海洋局第一海洋研究所基本科研业务专项资金项目(2012T08,GY2013G28) （2012T08,GY2013G28）
国家基金委-山东省联合基金项目“海洋生态与环境科学”(U1406403) （U1406403）
青岛市创业创新领军人才项目计划(13-CX-28) （13-CX-28）
