生物技术通报2017，Vol.33Issue(9)：172-178,7.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0303
小桐子转化酶基因家族的生物信息学分析及表达特性
Genome-wide Identification and Expression Characteristics of Invertase Gene Family in Jatropha curcas
摘要
Abstract
Plant invertase is one of critical enzymes in sucrose metabolism and stress resistance formation. The 16 invertase genes were identified based on complete genome data of Jatropha curcas,then the evolutionary relationship,gene structure,physical and chemical characteristics,conserved motif,and expression feature were analyzed. The results showed that invertase gene family in J. curcas was clustered to three subfamilies according to phylogenetic analysis. The α subgroup of neutral/alkaline invertases and acidic invertase gene owned 6-7 exons,and β subgroup owned 4-5 exons in gene structure. Signal peptides in N-terminal and conserved motifs of -NDPNG/A- and -MWECV/P-belonging to GH32 family were identified in acidic invertase protein sequences. qRT-PCR analysis revealed that JcC-INVD expressed differently in different tissues,with abundantly in cotyledon,but scarcely in root,and remarkably cold-induced expression in leaf.关键词
小桐子/转化酶/基因家族/表达分析Key words
Jatropha curcas/invertase/gene family/expression analysis引用本文复制引用
王海波,辛胡,刘潮,唐利洲..小桐子转化酶基因家族的生物信息学分析及表达特性[J].生物技术通报,2017,33(9):172-178,7.基金项目
国家自然科学基金项目(31460179) （31460179）
