特产研究2017，Vol.39Issue(2)：1-4,8,5.DOI:10.16720/j.cnki.tcyj.2017.02.001
人参糖蛋白的结构及其镇静安眠作用研究
Study on the Structure and Sedative Hypnotic Effects of Panax ginseng Glycoprotein
摘要
Abstract
Study on the structure and sedative hypnotic effects of the glycoprotein,which was obtained from water extract of Panax ginseng.Its neutral sugar content was 37.27％,protein content was 53.98％,and acid sugar content was 10.45％.And it was a mixture which weight average molecular weight was 17 73 1u analyzed by HPLC and GPC software.The sugar chains were consist of 6 kinds of monosaccharide,non reducing ends were Rha、Man、Glc and Gal,which was mainly Glc,and Rha only exist in the form of non reducing ends in the sugar chain.The backbone were composed of (1 →2) and (1→4)-linked GalA,and (1 →4)-linked Glc,which was-mainly (1 →4)-linked Glc,and there were also a small amount of branches which were (1 → 3,4),(1 →4,6) and (1 →3,6)-linked Man.The result of β-elimination reaction showed it was O-linked glycoprotein.The GalNAc connected to amino acid by (1 →4)-linkage.The ginseng glycoprotein (i.p.,at the dose of 20mg/kg) could significantly reduce the number of activities in mice,and had synergistic effect on pentobarbital sodium of under threshold dose,can significantly reduced the sleep latency (P ＜ 0.05),prolong the sleeping time of mice.So the ginseng glycoprotein showed sedative hypnotic effects.关键词
人参/糖蛋白/自主活动/延长睡眠时间Key words
Panax ginseng/glycoprotein/locomotor activity/prolong sleeping time分类
医药卫生引用本文复制引用
王颖,陈英红,徐宏,姜瑞芝,罗浩铭..人参糖蛋白的结构及其镇静安眠作用研究[J].特产研究,2017,39(2):1-4,8,5.基金项目
国家自然科学基金项目(81473326) （81473326）
