特产研究2017，Vol.39Issue(2)：5-8,4.DOI:10.16720/j.cnki.tcyj.2017.02.002
山兰种子无菌萌发及形态变化研究
Study on Asymbictic Germination and Morphology Change of Oreorchis patens (Lindl.) Lindl.Seeds
张正海 1张悦 1张亚玉 1孙海 1邵财 1王秋霞2
作者信息
- 1. 中国农业科学院特产研究所,长春130112
- 2. 集安市经济开发区农业技术推广站,吉林集安134200
- 折叠
摘要
Abstract
This paper presented the culture conditions for the asymbiotic germination of Oreorchispatens(Lindl.)Lindl.seeds and the morphology change during germination.Methods:The effcet of different ratios of 6-BA,NAA on development pathway of corm,salt strength of MS medium and light intensity on germination of seeds were observed,The morphology change was also investigated during seed germinating.Dark condition was suitable for seeds germination,the optimum medium for seeds germination was MS+NAA 0.5 mg/L+6-BA 2.0mg/L,the germination process was divided into 6 stages:embryonic cell absorption water and swell stage,heart-shaped embryo stage,torpedo-shape stage,cotyledon embryo stage,protocorm formation stage and corm formation stage.关键词
山兰/种子/无菌萌发/形态变化Key words
Oreorchis patens(Lindl.)Lindl./seeds/asymbictic germination/morphology change分类
农业科技引用本文复制引用
张正海,张悦,张亚玉,孙海,邵财,王秋霞..山兰种子无菌萌发及形态变化研究[J].特产研究,2017,39(2):5-8,4.
