天然产物研究与开发2017，Vol.29Issue(8)：1265-1269,5.DOI:10.16333/j.1001-6880.2017.8.001
葡萄籽原花青素通过Nrf2/ARE信号通路抗高糖诱导的HUVEC-12细胞氧化损伤
Protective Effects of Grape-Seed Proanthocyanidin Extract against High Glucose-induced Injury in HUVEC-12 Cells via Nrf2/ARE Pathway
摘要
Abstract
The objective of this study is to explore the protective effect and mechanisms of grape seed proanthocyanidin extract (GSPE) against oxidative stress of HUVEC-12 cells induced by high glucose.HUVEC-12 cell model induced by high glucose was established.The proliferation of HUVEC-12 cells were evaluated.The levels of reactive oxygen species (ROS),lactate dehydrogenase (LDH) and superoxide superoxide dismutase (SOD) were assayed.Both mRNA expression levels and protein expression levels of Nrf2/ARE signaling pathway related genes were detected.The results showed that GSPE intervention can improve the antioxidant capacity of HUVEC-12 cells significantly,decrease the ROS levels in cells induced by high glucose,improve the SOD activity and the cell vitality in a dose-dependent effect (P ＜ 0.05).The analysis showed that GSPE can also increase the mRNA expression levels of Nrf2,GSH-Px,HO-1,γ-GCS,NQO1 and protein contents of HO-1,NQO1.The results indicated that GSPE may protect against the oxidative damage of HUVEC12 cells induced by high glucose via the activation of Nrf2/ARE signal pathway.关键词
葡萄籽原花青素/高糖/Nrf2/ARE信号通路/HUVEC-12细胞Key words
grape seed proanthocyanidin extract/high glucose/Nrf2/ARE pathway/HUVEC-12 cell分类
医药卫生引用本文复制引用
张婷,刘瑞,宋小锋,张慧..葡萄籽原花青素通过Nrf2/ARE信号通路抗高糖诱导的HUVEC-12细胞氧化损伤[J].天然产物研究与开发,2017,29(8):1265-1269,5.基金项目
河南省科技攻关项目(172102310311) （172102310311）
新乡医学院三全学院创新团队(STD201603) （STD201603）
