天然产物研究与开发2017，Vol.29Issue(8)：1290-1295,6.DOI:10.16333/j.1001-6880.2017.8.005
两面针的愈伤组织诱导与分化
Induction and Differentiation of Calli from Zanthoxylum nitidum
汤丽云 1何蔚思 2何国振3
作者信息
- 1. 华南农业大学生命科学学院,广州510642
- 2. 新南威尔士大学理学院,悉尼NSW2025,澳大利亚
- 3. 广州中医药大学中药学院,广州510006
- 折叠
摘要
Abstract
Zanthoxylum nitidum is a woody liana plant with high value of medicine,but its resources is shortage.To recover the resources of Z.nitidum,germchits should be obtained by tissue culture.The results showed that the optimal medium for callus induction from explant of sterile stem segment was MS + 4.0 mg/L KT + 2.0 mg/L NAA with induction rate of 74.84％.The optimal medium for bud differentiation was MS + 6.0 mg/L KT + 0.2 mg/L IBA.The bud differentiation rate was 36.46％ after two times of differentiation culture,and 3.30 buds were regenerated from a piece of callus.The quantity and quality of regenerated bud could be improved and increased by proliferation culture.The optimal medium for bud proliferation was MS + 4.0 mg/L KT + 0.8 mg/L NAA,the rate of bud proliferation reached 5.00 after 8 weeks culture.The optimal medium for root differentiation was 1/2MS + 0.2 mg/L KT + 1.0 mg/L IBA with highest differentiation rate of 66.75％.A tissue culture system with high proliferation rate was established by successfully differentiating buds and roots from calli of Z.nitidum.关键词
两面针/组织培养/外植体/愈伤组织/分化Key words
Zanthoxylum nitidum/tissue culture/explant/callus/differentiation分类
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汤丽云,何蔚思,何国振..两面针的愈伤组织诱导与分化[J].天然产物研究与开发,2017,29(8):1290-1295,6.
