天然产物研究与开发2017，Vol.29Issue(8)：1296-1301,6.DOI:10.16333/j.1001-6880.2017.8.006
对羟基苯甲醛对神经元缺氧所致钙超载的抑制作用
Inhibition Effect of 4-Hydroxybenzaldehydeon Calcium Channels in Neurons
摘要
Abstract
The rat primary cortical neurons oxygen-glucose deprivation/reintroduction (OGD/Rep) injury model was duplicated and was used to study the inhibitory effect of 4-hydroxybenzaldehyde on calcium channel.The cell viability was texted by MTT assay.The NO release was texted by nitric acid reductase assay.Intracellular calcium concentration was texted by flow cytometry and the protein and mRNA expressions of L-type calcium channel,NMDA receptor-type calcium channel and TRPM7 channel were texted by Western Blot and RT-PCR.The results indicated 4-hydroxybenzaldehydecan elevate survival rate of the primary cortical neuron which were affected by OGD/Rep injury,decrease NO release,reduce intracellular calcium iron concentration of the primary cortical neurons,as well as lower L channel protein expression.关键词
对羟基苯甲醛/OGD/Rep/原代皮层神经元/钙通道Key words
4-hydroxybenzaldehyde/OGD/Rep/primary cortical neurons/calcium channel分类
医药卫生引用本文复制引用
周晓楠,宋雪兰,柳弯,解宇环,宋波,何芳雁..对羟基苯甲醛对神经元缺氧所致钙超载的抑制作用[J].天然产物研究与开发,2017,29(8):1296-1301,6.基金项目
国家自然科学基金(81560664,81660677) （81560664,81660677）
云南省应用基础研究项目(2015FB205(-027)) （2015FB205(-027）
