天然产物研究与开发2017,Vol.29Issue(8):1339-1342,4.DOI:10.16333/j.1001-6880.2017.8.014
鄂尔多斯高原钝顶节旋藻SOD特性研究
Characteristics of SOD in Arthrospira platensis from Erdos Plateau
摘要
Abstract
In this study,the pyrogallol auto-oxidation method was used to investigate the characteristics of superoxide dismutase (SOD) of Arthrospira platensis from the Erdos Plateau.The maximum absorption wavelength for SOD was 320 nm in the ultraviolet range.The optimal pH in a Tris-HCl buffer and the optimal temperature for SOD were 8.2 and 25 ℃,respectively.The SOD activity was higher in a pH range of 6-10.Superoxide dismutase activity decreased at temperatures higher than 40 ℃ for more than 20 minutes and higher than 35 ℃ for more than 60 minutes.Similarly,SOD activity decreased at room temperature in light after a period of more than 2 days,almost not at all after seven days at room temperature in light,and exhibited absolutely no change at room temperature without light after nine days.关键词
钝顶节旋藻/超氧化物岐化酶/特性Key words
Arthrospira platensis/superoxide dismutase/characteristics分类
生物科学引用本文复制引用
包玉兰,敖恩宝力格,乔辰,巩东辉..鄂尔多斯高原钝顶节旋藻SOD特性研究[J].天然产物研究与开发,2017,29(8):1339-1342,4.基金项目
国家自然科学基金(30460104,31660151) (30460104,31660151)
内蒙古自然科学基金(MS20160334) (MS20160334)