天然产物研究与开发2017，Vol.29Issue(8)：1349-1354,6.DOI:10.16333/j.1001-6880.2017.8.016
正交实验法优化碱水解栀子提取物制取京尼平苷酸的工艺
Preparation of Geniposidic Acid by Alkaline Hydrolysis of Gardenia Extract
摘要
Abstract
The conditions for the preparation of geniposidic acid from Gardenia extract by alkaline hydrolysis were optimized in this study.The effects of hydrolysis temperature,adding amount of NaOH,solid to liquid ratio,hydrolysis time were investigated and orthogonal experiments of L9 (34) were carried out.The optimal conditions were obtained as follow:hydrolysis temperature was 70 ℃,adding amount of NaOH was 14.0 mL,solid to liquid ratio was 1∶30 g/mL,hydrolysis time was l0 min.Under these conditions,the yield of geniposidic acid was 11.53％.The purity of geniposidic acid was increased to 45.33％ after static adsorption by activated carbon.关键词
栀子提取物/京尼平苷/京尼平苷酸/碱水解/活性炭Key words
Gardenia extract/geniposide/geniposidic acid/alkaline hydrolysis/activated carbon分类
医药卫生引用本文复制引用
赵帅,刘磊磊,王欢,郭婕,彭密军..正交实验法优化碱水解栀子提取物制取京尼平苷酸的工艺[J].天然产物研究与开发,2017,29(8):1349-1354,6.基金项目
湖南省自然科学基金(14JJ6037) （14JJ6037）
湖南省教育厅一般项目(15C1116) （15C1116）
吉首大学人才引进专项(jsdxrcyjkyxm201301) （jsdxrcyjkyxm201301）
