天然产物研究与开发2017，Vol.29Issue(8)：1396-1402,1379,8.DOI:10.16333/j.1001-6880.2017.8.023
丹参中丹参酮ⅡA和丹酚酸B的提取与纯化工艺研究
Extraction and Purification of Tanshinone Ⅱ A and Salvianolic Acid B from Salvia Miltiorrhiza Bunge
摘要
Abstract
A method for simultaneous extraction of tanshinone ⅡA and salvianolic acid B from the SalviamiltiorrhizaBunge and the purification of salvianolic acid B was developed.The extraction process was optimized by orthogonal design with the content of tanshinone ⅡA and slvianolic acid B as evaluation indexes.The optimal extraction process was as follows:S.miltiorrhizapowder was extracted by 8 times of 70％ ethanol with at 85 ℃ (reflux) for three times in 1 h.The extraction rate of tanshinone ⅡA was higher than 90％,and the extraction rate of salvianolic acid B was higher than 80％.The extract was dissolved in hot waterand filtered,the filtrate was concentrated to 1 mL of a solution containing about 0.5 g of crude drugwith the volume of 100 mL was loaded on the D101macroporous resin column with the sample flow rate of 2 BV/h,and then column was washed by 6 BV of water,8 BV of 30％ ethanol with the flow rate of 4 BV/h for removing impurities,10 BV of 60％ ethanol with 2 BV/h flow rate for purifying,to obtain more than 75％ purity of salvianolic acid B,verification experiments showed that the process was stable and feasible;Finally,after washing and methanol recrystallization,more than 99％ purity salvianolic acid B was obtained,the total yield of about 2.86％ (based onthe dried rhizomes).关键词
丹参酮ⅡA/丹酚酸B/提取/大孔树脂/纯化Key words
tanshinoneⅡA/salvianolicacid B/extraction/macroporousresin/purification分类
医药卫生引用本文复制引用
熊加伟,葛松兰,马磊..丹参中丹参酮ⅡA和丹酚酸B的提取与纯化工艺研究[J].天然产物研究与开发,2017,29(8):1396-1402,1379,8.基金项目
上海自然科学基金(15ZR1408800) （15ZR1408800）
上海浦江人才计划(15PJD012) （15PJD012）
