武警医学2017，Vol.28Issue(6)：600-604,608,6.
IFN-γ预处理骨髓间充质干细胞及其抑制小胶质细胞活化作用的影响
Inhibitory effects of IFN-γ pretreated bone marrow mesenchymal stem cells on lipopolysaccharide-induced microglia activation
杜春彦 1杨静 1王晓晨 1冯泽国 1郭全义 2戴鑫3
作者信息
- 1. 100853北京,解放军总医院麻醉科
- 2. 100853北京,解放军总医院骨科
- 3. 100853北京,解放军总医院实验动物中心
- 折叠
摘要
Abstract
Objective To explore the inhibitory effect of pre-treatment of one marrow mesenchymal stem cells (BMMSCs) with interferon-γand IFN-γ on the activation of microglia(MG) induced by lipopolysaccharide(LPS).Methods BMMSCs were isolated and cultured using the whole bone marrow adherence method.Then,100ng/ml IFN-γwas used to induce the activation of BMMSCs and10 μg/ml lipopolysaccharide was used as the inflammatory stimulating factor to induce the activation of microglia.After isolation and purification,microglia were seeded in 6-well plates at the density of 1 × 105/well and BMMSCs were co-cultured with microglia with the cell contact cell method for 24 h in the following groups:B group(BMMSCs group),L + B group(LPS + BMMSCs group),I + B group (IFN-γ pretreatment BMMSCs group),L + I + B group(LPS + IFN-γpretreatment BMMSCs group),M group(microglia group),L + M group(LPS + microglia group),B + L + M group(BMMSCs + LPS + microglia group),and I + B + L + M group(BMMSCs pretreated with IFN-γ+ LPS + microglia group).24 hours later,the expressions of TNF-α 、IL-1 βin cell culture supernatants were detected by ELISA kit and the expression of microglia surface marker CD68 was detected by immunofluorescence.Results In the L + M group,LPS promoted the expression of CD68 and the production of TNF-α and IL-1β in microglial cells.The expression of CD68 and the production of TNF-α and IL-1β in B + L + M group and I + B + L + M group were lower than in L + M group,and there was statistically significant difference between the B + L + M group and the I + B + L + M group (P ＜ 0.05).Conclusions Both BMMSCs and IFN-γ pretreatment of BMMSCs can inhibit the activation of LPS-induced microglia by direct cell contact.After IFN-γ pretreatment,BMMSCs could more strongly inhibit the activity of microglia.关键词
骨髓间充质干细胞/小胶质细胞/活化/预处理/干扰素γKey words
bone marrow mesenchymal stem cells/microglia/activation/pretreatment/IFN-γ分类
基础医学引用本文复制引用
杜春彦,杨静,王晓晨,冯泽国,郭全义,戴鑫..IFN-γ预处理骨髓间充质干细胞及其抑制小胶质细胞活化作用的影响[J].武警医学,2017,28(6):600-604,608,6.
